Three-dimensional reconstructions of extracellular matrix polymers using automated electron tomography
Abstract
The extracellular matrix is an intricate network of macromolecules which provides support for cells and a framework for tissues. The detailed structure and organisation of most matrix polymers is poorly understood. These polymers have a complex ultrastructure, and it has proved a major challenge both to define their structural organisation and to relate this to their biological function. However, new approaches using automated electron tomography are beginning to reveal important insights into the molecular assembly and structural organisation of two of the most abundant polymer systems in the extracellular matrix. We have generated three-dimensional reconstructions of collagen fibrils from bovine cornea and fibrillin microfibrils from ciliary zonules. Analysis of these data has provided new insights into the organisation and function of these large macromolecular assemblies.
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At the supra-molecular level, the initial building block is a 4-8 nm wide microfibril with a left-handed twist of the constituent molecules.
“At the supra-molecular level, the initial building block is a 4–8 nm wide microfibril (Baldock et al., 2002) with a left-handed twist of the constituent molecules (Fig. 2).”
From Corneal structure and transparency (2015) · cites this paper as Baldock et al., 2002
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Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
- Authors
- Baldock, C., Gilpin, C. J., Koster, A. J., Ziese, U., Kadler, K. E., Kielty, C. M., & Holmes, D. F.
- Affiliations
- University of Manchester (GB)
- Utrecht University (NL)
- Corresponding author
- Baldock, C.
- Venue
- Journal of Structural Biology
- Publisher
- Elsevier BV
- Published
- 2002-04
- Volume
- 138
- Issue
- 1-2
- Pages
- 130-136
- ISSN
- 1047-8477 (ISSN-L)
- OpenAlex
- W2126180289
- Cited by
- 27
- References
- 0
- Citations by year
- 2020: 1 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 1
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Structural Biology (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Keywords
- extracellular matrix, collagen fibrils, 3D reconstruction, cornea, ciliary zonule, macromolecular complexes, ultrastructure
- MeSH terms
- Fibrillins, Animals, Cattle, Cornea, Extracellular Matrix, Microfilament Proteins, Microscopy, Electron, Tomography, X-Ray Computed, Microfibrils, Imaging, Three-Dimensional, Fibrillar Collagens
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