The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Glucosamine inhibits IL-1β-induced NFκB activation in human osteoarthritic chondrocytes
Open accessAbstract
Abstract Objective : Glucosamine sulfate (GS) is a commonly used drug for the treatment of osteoarthritis. The mechanism of the action of this drug does, however, remain to be elucidated. In human osteoarthritic chondrocytes (HOC) stimulated with a proinflammatory cytokine, we studied whether GS could modify the NFκB activity and the expression of COX-2, a NFκB-dependent gene. Methods : Using HOC in culture stimulated with interleukin-1 β (IL-1β), the effects of GS on NFκB activation, nuclear translocation of NFκB/Rel family members, COX-1 and COX-2 expressions and syntheses and prostaglandin E2 (PGE2) concentration were studied. Results : GS significantly inhibited NFκB activity in a dose-dependent manner, as well as the nuclear translocation of p50 and p65 proteins. Furthermore, GS-preincubated IL-1β-stimulated HOC showed an increase in IκBα in the cell cytoplasm in comparison with HOC incubated with IL-1β alone. GS also inhibited the gene expression and the protein synthesis of COX-2 induced by IL-1β, while no effect on COX-1 synthesis was seen. GS also inhibited the release of PGE2 to conditioned media of HOC stimulated with IL-1β. Conclusions : GS inhibits the synthesis of proinflammatory mediators in HOC stimulated with IL-1β through a NFκB-dependent mechanism. Our study further supports the role of GS as a symptom- and structure-modifying drug in the treatment of OA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
NF-κB: a key role in inflammatory diseases
Tak, P. P., & Firestein, G. S. (2001) · Journal of Clinical Investigation · doi:10.1172/jci11830 · Cited by 3,918
Kelley, W. N. (1985) · Virtual Defense Library (Ministerio de Defensa) · Cited by 1,670
ARTHRITIS AND ALLIED CONDITIONS
BERNARD I. COMROE, T. L., & HOLLANDER, J. L. (1949) · The American Journal of the Medical Sciences · doi:10.1097/00000441-194911000-00026 · Cited by 1,581
Reginster, J. Y., Deroisy, R., Rovati, L. C., Lee, R. L., Lejeune, E., Bruyere, O., Giacovelli, G., Henrotin, Y., Dacre, J. E., & Gossett, C. (2001) · The Lancet · doi:10.1016/s0140-6736(00)03610-2 · Cited by 1,234
Series Introduction: The transcription factor NF-κB and human disease
Baldwin, A. S. (2001) · Journal of Clinical Investigation · doi:10.1172/jci11891 · Cited by 990
Arthritis and Allied Conditions: A Textbook of Rheumatology.
(1991) · Annals of Internal Medicine · doi:10.7326/0003-4819-114-9-820_6 · Cited by 766
Isolation of an interleukin‐1‐like factor from human joint effusions
Wood, D. D., Ihrie, E. J., Dinarello, C. A., & Cohen, P. L. (1983) · Arthritis & Rheumatism · doi:10.1002/art.1780260806 · Cited by 445
Evidence for Involvement of NF-κB in the Transcriptional Control of COX-2 Gene Expression by IL-1β
Newton, R., Kuitert, L. M. E., Bergmann, M., Adcock, I. M., & Barnes, P. J. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7064 · Cited by 389
Arthritis and Allied Conditions: A Textbook of Rheumatology
Smyth, C. J. (1985) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.1985.03360180182053 · Cited by 322
Cited by
NF-κB as a potential therapeutic target in osteoarthritis and rheumatoid arthritis
Roman-Blas, J. A., & Jimenez, S. A. (2006) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2006.04.008 · Cited by 688
Cell signaling, the essential role of O-GlcNAc
Zachara, N. E., & Hart, G. W. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2006.04.007 · Cited by 386
Herrero‐Beaumont, G., Ivorra, J. A. R., del Carmen Trabado, M., Blanco, F. J., Benito, P., Martín‐Mola, E., Paulino, J., Marenco, J. L., Porto, A., Laffon, A., et al. (2007) · Arthritis & Rheumatism · doi:10.1002/art.22371 · Cited by 287
Self-citationLiu, W., Liu, A., Li, X., Sun, Z., Sun, Z., Liu, Y., Wang, G., Huang, D., Xiong, H., Yu, S., et al. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.06.012 · Cited by 107
SCHULZE‐TANZIL, G., MOBASHERI, A., SENDZIK, J., JOHN, T., & SHAKIBAEI, M. (2004) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1329.067 · Cited by 92
Metadata
- Authors
- Largo, R., Alvarez-Soria, M. A., Dı́ez-Ortego, I., Calvo, E., Sánchez-Pernaute, O., Egido, J., & Herrero-Beaumont, G.
- Venue
- Osteoarthritis and Cartilage
- Publisher
- Elsevier BV
- Published
- 2003-04
- Volume
- 11
- Issue
- 4
- Pages
- 290-298
- ISSN
- 1063-4584 (ISSN-L)
- OpenAlex
- W2087492003
- Cited by
- 386
- References
- 27
- FWCI
- 8.63
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Glucosamine inhibits IL-1β-induced NFκB activation in human osteoarthritic chondrocytes. https://app.cochat.ai/papers/10.1016/s1063-4584(03)00028-1
- The paper
- Largo, R., Alvarez-Soria, M. A., Dı́ez-Ortego, I., Calvo, E., Sánchez-Pernaute, O., Egido, J., & Herrero-Beaumont, G. (2003). Glucosamine inhibits IL-1β-induced NFκB activation in human osteoarthritic chondrocytes. Osteoarthritis and Cartilage. https://doi.org/10.1016/s1063-4584(03)00028-1
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
