The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A general approach for the generation of orthogonal tRNAs
Open accessAbstract
BACKGROUND The addition of new amino acids to the genetic code of Escherichia coli requires an orthogonal suppressor tRNA that is uniquely acylated with a desired unnatural amino acid by an orthogonal aminoacyl-tRNA synthetase. A tRNA(Tyr)(CUA)-tyrosyl-tRNA synthetase pair imported from Methanococcus jannaschii can be used to generate such a pair. In vivo selections have been developed for selecting mutant suppressor tRNAs with enhanced orthogonality, which can be used to site-specifically incorporate unnatural amino acids into proteins in E. coli. RESULTS A library of amber suppressor tRNAs derived from M. jannaschii tRNA(Tyr) was generated. tRNA(Tyr)(CUA)s that are substrates for endogenous E. coli aminoacyl-tRNA synthetases were deleted from the pool by a negative selection based on suppression of amber nonsense mutations in the barnase gene. The remaining tRNA(Tyr)(CUA)s were then selected for their ability to suppress amber nonsense codons in the beta-lactamase gene in the presence of the cognate M. jannaschii tyrosyl-tRNA synthetase (TyrRS). Four mutant suppressor tRNAs were selected that are poorer substrates for E. coli synthetases than M. jannaschii tRNA(Tyr)(CUA), but still can be charged efficiently by M. jannaschii TyrRS. CONCLUSIONS The mutant suppressor tRNA(Tyr)(CUA) together with the M. jannaschii TyrRS is an excellent orthogonal tRNA-synthetase pair for the in vivo incorporation of unnatural amino acids into proteins. This general approach may be expanded to generate additional orthogonal tRNA-synthetase pairs as well as probe the interactions between tRNAs and their cognate synthetases.
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Cites
A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins
Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989) · Science · doi:10.1126/science.2649980 · Cited by 1,019 · cited 1×
“In vitro methods have been developed to site-speci¢cally introduce unnatural amino acids into proteins by suppression of amber mutations with chemically acylated tRNAs [ 1 ,2].”
MethodSelf-citationUniversal rules and idiosyncratic features in tRNA identity
Giege, R., Sissler, M., & Florentz, C. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.22.5017 · Cited by 769
Biosynthetic site-specific incorporation of a non-natural amino acid into a polypeptide
Bain, J. D., Diala, E. S., Glabe, C. G., Dix, T. A., & Chamberlin, A. R. (1989) · Journal of the American Chemical Society · doi:10.1021/ja00202a052 · Cited by 250
Cited by
Adding New Chemistries to the Genetic Code
Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Self-citationAddition of p-Azido-l-phenylalanine to the Genetic Code of Escherichia coli
Chin, J. W., Santoro, S. W., Martin, A. B., King, D. S., Wang, L., & Schultz, P. G. (2002) · Journal of the American Chemical Society · doi:10.1021/ja027007w · Cited by 721
Self-citationA chemical toolkit for proteins — an expanded genetic code
Xie, J., & Schultz, P. G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2005 · Cited by 408
Self-citationLabeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114
Genetic Incorporation of Unnatural Amino Acids into Proteins in Mycobacterium tuberculosis
Wang, F., Robbins, S., Guo, J., Shen, W., & Schultz, P. G. (2010) · PLoS ONE · doi:10.1371/journal.pone.0009354 · Cited by 64
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Metadata
- Authors
- Wang, L., & Schultz, P. G.
- Venue
- Chemistry & Biology
- Publisher
- Elsevier BV
- Published
- 2001-09
- Volume
- 8
- Issue
- 9
- Pages
- 883-890
- ISSN
- 1074-5521 (ISSN-L)
- OpenAlex
- W2103563524
- Cited by
- 226
- References
- 3
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- bronze
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