Cited by 154Advanced Biosensing Techniques and ApplicationsComputational Drug Discovery MethodsChemical Synthesis and Analysis

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New assay technologies for high-throughput screening

Silverman, Lauren, Campbell, Robert, Broach, James R·1998·Current Opinion in Chemical Biology

DOI 10.1016/s1367-5931(98)80015-x

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Abstract

The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to interference from test compounds needed to function in a high-throughput screening mode. Similarly, novel cell-based assays are now being adapted for high-throughput screening, providing for in situ analysis of a variety of biological targets. Finally, recent advances in assay miniaturization mark a transition to ultra high-throughput screening, ensuring that identification of lead compounds will not be the rate-limiting step in finding new drugs.

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Authors: Silverman, L., Campbell, R., & Broach, J. R.
Affiliations: Princeton University (US)
Corresponding author: Broach, James R
ORCID: Campbell, Robert
Broach, James R
Venue: Current Opinion in Chemical Biology
Publisher: Elsevier BV
Published: 1998-06
Volume: 2
Issue: 3
Pages: 397-403
ISSN: 1367-5931 (ISSN-L)
DOI: 10.1016/s1367-5931(98)80015-x
OpenAlex: W2092399057
Cited by: 151
References: 55
Citations by year: 2025: 2 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 2 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 3
FWCI: 0.95
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Current Opinion in Chemical Biology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Drug Discovery › Computational Drug Discovery Methods
Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
Keywords: high-throughput screening, fluorescence correlation spectroscopy, cell-based assays, assay miniaturization, ultra-high-throughput screening, lead compound identification, early drug discovery
MeSH terms: Animals, Cell Line, Cell-Free System, Chemistry, Organic, Drug Evaluation, Preclinical, Energy Transfer, Mammals, Miniaturization, Spectrometry, Fluorescence, Transfection, Drug Design, Genes, Reporter

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