Methods to induce primary and secondary traumatic damage in organotypic hippocampal slice cultures
Abstract
Organotypic brain slice cultures have been used in a variety of studies on neurodegenerative processes [K.M. Abdel-Hamid, M. Tymianski, Mechanisms and effects of intracellular calcium buffering on neuronal survival in organotypic hippocampal cultures exposed to anoxia/aglycemia or to excitotoxins, J. Neurosci. 17, 1997, pp. 3538-3553; D.W. Newell, A. Barth, V. Papermaster, A.T. Malouf, Glutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures, J. Neurosci. 15, 1995, pp. 7702-7711; J.L. Perez Velazquez, M.V. Frantseva, P.L. Carlen, In vitro ischemia promotes glutamate mediated free radical generation and intracellular calcium accumulation in pyramidal neurons of cultured hippocampal slices, J. Neurosci. 23, 1997, pp. 9085-9094; L. Stoppini, L.A. Buchs, D. Muller, A simple method for organotypic cultures of nervous tissue, J. Neurosci. Methods 37, 1991, pp. 173-182; R.C. Tasker, J.T. Coyle, J.J. Vornov, The regional vulnerability to hypoglycemia induced neurotoxicity in organotypic hippocampal culture: protection by early tetrodotoxin or delayed MK 801, J. Neurosci. 12, 1992, pp. 4298-4308.]. We describe two methods to induce traumatic cell damage in hippocampal organotypic cultures. Primary trauma injury was achieved by rolling a stainless steel cylinder (0.9 g) on the organotypic slices. Secondary injury was followed after dropping a weight (0.137 g) on a localised area of the organotypic slice, from a…
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Toxic levels of glutamate induce morphological changes in neurons, manifesting in deformed dendrites and expanded rounded neurons.
“These toxic levels of glutamate induce morphological changes in neurons, manifesting in deformed dendrites and expanded rounded neurons (Reynolds’ and Hastings, 1995; Jellinger, 1999; Adamchik et al., 2000; Robert et al., 2002; Youdim et al., 2002; García et al., 2005), showing degenerative cell (Kang et al., 2020; Erkartal et al., 2025) and neuronal death (Weidinger et al., 2023).”
From Toxic Concentrations of Glutamate Induce Ferroptosis without Affecting Mitochondrial Respiration in Rodent Brain Slice Cultures (2026) · cites this paper as Adamchik et al., 2000
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Eljaschewitsch, E., Witting, A., Mawrin, C., Lee, T., Schmidt, P. M., Wolf, S., Hoertnagl, H., Raine, C. S., Schneider-Stock, R., Nitsch, R., et al. (2006) · Neuron · doi:10.1016/j.neuron.2005.11.027 · Cited by 445 · see citing works
Vaglio-Garro, A., Theuermann, M., Sweis, K. E., Zavadskis, S., Kokas, M., Környei, Z., Karas, M., Tretter, L., Komlódi, T., Weidinger, A., et al. (2026) · eneuro · doi:10.1523/eneuro.0439-25.2026
- Authors
- Adamchik, Y., Frantseva, M. V., Weisspapir, M., Carlen, P. L., & Perez Velazquez, J. L.
- Affiliations
- University of Toronto (CA)
- ORCID
- Carlen, P.L.
- Venue
- Brain Research Protocols
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 5
- Issue
- 2
- Pages
- 153-158
- ISSN
- 1385-299X (ISSN-L)
- OpenAlex
- W2020463662
- Cited by
- 101
- References
- 0
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 4 · 2021: 1 · 2019: 1 · 2018: 3 · 2017: 4 · 2016: 3 · 2015: 5 · 2014: 2 · 2013: 5 · 2012: 5
- FWCI
- 2.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research Protocols (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- traumatic brain injury, organotypic hippocampal slice cultures, primary trauma, secondary injury, mechanical damage, cell death, neuronal viability, propidium iodide, excitotoxicity, neurodegeneration, hypoglycemia, oxygen-glucose deprivation, excitatory amino acid toxicity, calcium buffering, tetrodotoxin, MK-801, neuroprotection
- MeSH terms
- Animals, Brain Injuries, Hippocampus, Male, Neurology, Neurons, Organ Culture Techniques, Temperature, Time Factors, Wounds, Nonpenetrating, Cell Death, Rats, Wistar, Rats
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