The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ?
Open accessAbstract
Evidence is presented that xylose metabolism in the anaerobic cellulolytic fungus Piromyces sp. E2 proceeds via a xylose isomerase rather than via the xylose reductase/xylitol-dehydrogenase pathway found in xylose-metabolising yeasts. The XylA gene encoding the Piromyces xylose isomerase was functionally expressed in Saccharomyces cerevisiae. Heterologous isomerase activities in cell extracts, assayed at 30 degrees C, were 0.3-1.1 micromol min(-1) (mg protein)(-1), with a Km for xylose of 20 mM. The engineered S. cerevisiae strain grew very slowly on xylose. It co-consumed xylose in aerobic and anaerobic glucose-limited chemostat cultures at rates of 0.33 and 0.73 mmol (g biomass)(-1) h(-1), respectively.
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Metadata
- Authors
- KUYPER, M., HARHANGI, H., STAVE, A., WINKLER, A., JETTEN, M., DELAAT, W., DENRIDDER, J., OPDENCAMP, H., VANDIJKEN, J., & PRONK, J.
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-10
- Volume
- 4
- Issue
- 1
- Pages
- 69-78
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W1861998278
- Cited by
- 366
- References
- 40
- FWCI
- 7.69
- Type
- journal article
- Language
- en
- Open access
- bronze
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- KUYPER, M., HARHANGI, H., STAVE, A., WINKLER, A., JETTEN, M., DELAAT, W., DENRIDDER, J., OPDENCAMP, H., VANDIJKEN, J., & PRONK, J. (2003). High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ? FEMS Yeast Research. https://doi.org/10.1016/s1567-1356(03)00141-7
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
