Young women partition fatty acids towards ketone body production rather than VLDL-TAG synthesis, compared with young men
Open accessAbstract
Before the menopause, women are relatively protected against CVD compared with men. The reasons for this sex difference are not completely understood, but hepatic fatty acid metabolism may play a role. The present study aimed to investigate the utilisation of plasma NEFA by the liver and to determine whether they are partitioned differently into ketone bodies and VLDL-TAG in healthy, lean young men and women. Volunteers were studied during a prolonged overnight fast (12-19 h) using an intravenous infusion of [U-¹³C]palmitate. After 12 h fasting, the women had a more advantageous metabolic profile with lower plasma glucose (P < 0·05) and TAG (P < 0·05) but higher plasma NEFA (P < 0·05) concentrations. Plasma 3-hydroxybutyrate (3-OHB) concentrations rose more in women than in men, and the transfer of ¹³C from [U-¹³C]palmitate to plasma [¹³C]3-OHB reached a plateau 6-7 h after the start of the infusion in women but was still increasing at 6 h in men. This implies a slower 3-OHB production rate and/or dilution by other precursor pools in men. In women, the high isotopic enrichment of plasma 3-OHB suggested that systemic plasma fatty acids were the major source of 3-OHB production. However, in men, this was not observed during the course of the study (P < 0·01). There were no sex differences for the incorporation of ¹³C into VLDL1- or VLDL2-TAG. The ability of young women to partition fatty acids towards ketone body production rather than VLDL-TAG may contribute to their more advantageous metabolic profile compared with young men.
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After an overnight fast, ketone bodies are in the micromolar range (~30 μM for women and ~60 μM for men), which is not detected by most assays.
“After an overnight fast, ketone bodies are in the micromolar range (∼30 μM for women and ∼60 μM for men), which is not detected by most assays (23).”
From Metabolic Flexibility as an Adaptation to Energy Resources and Requirements in Health and Disease (2018) · cites this paper as [23]
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- Authors
- Marinou, K., Adiels, M., Hodson, L., Frayn, K. N., Karpe, F., & Fielding, B. A.
- Affiliations
- National and Kapodistrian University of Athens (GR)
- University of Gothenburg (SE)
- Churchill Hospital (GB)
- Corresponding author
- Fielding, Barbara A.
- Venue
- British Journal Of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 2011-01-21
- Volume
- 105
- Issue
- 6
- Pages
- 857-865
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W2126260552
- Cited by
- 75
- References
- 28
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 3 · 2020: 4 · 2019: 6 · 2018: 7 · 2017: 5 · 2016: 5 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Surrey Research Insight Open Access (The University of Surrey) (submittedVersion)
- Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
- Chalmers Research (Chalmers University of Technology) (submittedVersion)
- Funders
- European Atherosclerosis Society
- European Commission (LSHM‐CT‐2005‐018734)
- Novo Nordisk
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Diet and metabolism studies
- Keywords
- sex differences, ketone synthesis, beta-hydroxybutyrate, free fatty acids, palmitate, overnight fasting, cardiovascular risk, hepatic metabolism
- MeSH terms
- Adult, Blood Glucose, Carbon Isotopes, Dietary Fats, Fasting, Fatty Acids, Nonesterified, Female, Humans, Lipoproteins, VLDL, Liver, Male, Sex Factors, Triglycerides, Premenopause, Palmitic Acid, Postprandial Period, 3-Hydroxybutyric Acid, Young Adult
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