Synergistic attenuation of ovariectomy-induced bone loss by combined use of fish oil and 17 β-oestradiol
Open accessAbstract
Oestrogen and n-3 PUFA, especially EPA and DHA, have been reported to have beneficial effects on bone loss. Thus, the purpose of the present study was to investigate the synergistic bone-protective mechanism of combined treatments of EPA+DHA supplementation and oestrogen injection in ovariectomised rats. Rats were fed a modified American Institute of Nutrition-93G diet with 0 %, 1 % or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and after a 1-week recovery period rats were injected with either 17β-oestradiol-3-benzoate (E2) or maize oil for the last 3 weeks. Combined use of n-3 PUFA and E2 synergistically increased femoral cortical bone volume, bone mineral content and the bone expression of runt-related transcription factor 2 (RUNX2), but decreased the bone expression of IL-1β. Both n-3 PUFA and E2 decreased the bone expressions of IL-7, TNF-α and PPAR-γ, and increased the bone expression of oestrogen receptor-α. n-3 PUFA in the presence of E2 and E2 alone significantly decreased the bone expressions of IL-1β and IL-6 and increased the bone expression of RUNX2. E2 significantly decreased the serum levels of bone turnover markers and the bone expression of receptor activator of NF-κB ligand, but decreased the bone expression of osteoprotegerin. The combined use of n-3 PUFA and E2 exerted synergistic bone-protective efficacy through up-regulation of RUNX2, an essential transcription factor for bone formation, as well as the suppression of bone-resorbing cytokine IL-1β.
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n-3 PUFAs exert similar effects as oestrogen, preventing bone loss in ovariectomised rats.
“Furthermore, n-3 PUFAs have been shown to exert similar effects as the female sex hormone oestrogen, preventing bone loss in ovariectomised rats [113].”
From Potential Roles of n-3 PUFAs during Skeletal Muscle Growth and Regeneration (2018) · cites this paper as [113]
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- Authors
- Jin, Y., Lee, M., & Park, Y.
- Affiliations
- Hanyang University (KR)
- Sungshin Women's University (KR)
- Corresponding author
- Park, Yongsoon
- Venue
- British Journal Of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 2017-02-28
- Volume
- 117
- Issue
- 4
- Pages
- 479-489
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W2604589182
- Cited by
- 11
- References
- 39
- Citations by year
- 2024: 2 · 2022: 3 · 2021: 2 · 2020: 1 · 2018: 3
- FWCI
- 1.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- ovariectomy-induced bone loss, omega-3 fatty acids, eicosapentaenoic acid, docosahexaenoic acid, estradiol, Runx2, IL-1β, osteoprotegerin, RANKL, estrogen receptor alpha, bone turnover markers, postmenopausal osteoporosis, dietary fatty acid supplementation
- MeSH terms
- Animals, Bone Diseases, Metabolic, Bone Resorption, Drug Synergism, Estradiol, Female, Femur, Fish Oils, Osteogenesis, Ovariectomy, Tumor Necrosis Factor-alpha, Bone Density, Interleukin-6, Interleukin-7, Up-Regulation, NF-kappa B, Rats, Wistar, PPAR gamma, Estrogen Receptor alpha, Bone Density Conservation Agents, Core Binding Factor Alpha 1 Subunit, Osteoprotegerin, Interleukin-1beta
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