Cited by 11Bone health and osteoporosis researchFatty Acid Research and HealthBone Metabolism and Diseases

Synergistic attenuation of ovariectomy-induced bone loss by combined use of fish oil and 17 β-oestradiol

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Jin, Youri, Lee, Myoungsook, Park, Yongsoon·2017·British Journal Of Nutrition

Abstract

Oestrogen and n-3 PUFA, especially EPA and DHA, have been reported to have beneficial effects on bone loss. Thus, the purpose of the present study was to investigate the synergistic bone-protective mechanism of combined treatments of EPA+DHA supplementation and oestrogen injection in ovariectomised rats. Rats were fed a modified American Institute of Nutrition-93G diet with 0 %, 1 % or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and after a 1-week recovery period rats were injected with either 17β-oestradiol-3-benzoate (E2) or maize oil for the last 3 weeks. Combined use of n-3 PUFA and E2 synergistically increased femoral cortical bone volume, bone mineral content and the bone expression of runt-related transcription factor 2 (RUNX2), but decreased the bone expression of IL-1β. Both n-3 PUFA and E2 decreased the bone expressions of IL-7, TNF-α and PPAR-γ, and increased the bone expression of oestrogen receptor-α. n-3 PUFA in the presence of E2 and E2 alone significantly decreased the bone expressions of IL-1β and IL-6 and increased the bone expression of RUNX2. E2 significantly decreased the serum levels of bone turnover markers and the bone expression of receptor activator of NF-κB ligand, but decreased the bone expression of osteoprotegerin. The combined use of n-3 PUFA and E2 exerted synergistic bone-protective efficacy through up-regulation of RUNX2, an essential transcription factor for bone formation, as well as the suppression of bone-resorbing cytokine IL-1β.

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Authors
Jin, Y., Lee, M., & Park, Y.
Affiliations
  • Hanyang University (KR)
  • Sungshin Women's University (KR)
Corresponding author
Park, Yongsoon
Venue
British Journal Of Nutrition
Published
2017-02-28
Volume
117
Issue
4
Pages
479-489
ISSN
  • 0007-1145 (ISSN-L)
  • 1475-2662
OpenAlex
W2604589182
Cited by
11
References
39
Citations by year
2024: 2 · 2022: 3 · 2021: 2 · 2020: 1 · 2018: 3
FWCI
1.21
Type
journal article
Language
en
Open access
Locations
  • British Journal Of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Keywords
ovariectomy-induced bone loss, omega-3 fatty acids, eicosapentaenoic acid, docosahexaenoic acid, estradiol, Runx2, IL-1β, osteoprotegerin, RANKL, estrogen receptor alpha, bone turnover markers, postmenopausal osteoporosis, dietary fatty acid supplementation
MeSH terms
Animals, Bone Diseases, Metabolic, Bone Resorption, Drug Synergism, Estradiol, Female, Femur, Fish Oils, Osteogenesis, Ovariectomy, Tumor Necrosis Factor-alpha, Bone Density, Interleukin-6, Interleukin-7, Up-Regulation, NF-kappa B, Rats, Wistar, PPAR gamma, Estrogen Receptor alpha, Bone Density Conservation Agents, Core Binding Factor Alpha 1 Subunit, Osteoprotegerin, Interleukin-1beta

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