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Embryoid bodies: an in vitro model of mouse embryogenesis
Abstract
Embryonic stem (ES) cells are pluripotent cells isolated from the inner cell mass of blastocysts. ES cells are able to differentiate into the three primitive layers (endoderm, mesoderm and ectoderm) of the organism, including the germline. To study early stages of development, as well as to investigate the impact of a gene knock-out in vitro, ES cells are differentiated into three-dimensional structures called embryoid bodies, because of their ability to mimick post-implantation embryonic tissues. This review summarises the work on ES cell differentiation into haematopoietic and vascular cells, neuronal and glial cells, myocytes, and adipocytes, using this in vitro model of early embryogenesis. We also present the potential of this method to analyse the impact of genetic alterations in vitro.
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Pevny, L., Simon, M. C., Robertson, E., Klein, W. H., Tsai, S.-F., D'Agati, V., Orkin, S. H., & Costantini, F. (1991) · Nature · doi:10.1038/349257a0 · Cited by 1,308
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Metadata
- Authors
- Desbaillets, I., Ziegler, U., Groscurth, P., & Gassmann, M.
- Venue
- Experimental Physiology
- Publisher
- Wiley
- Published
- 2000-11
- Volume
- 85
- Issue
- 6
- Pages
- 645-651
- ISSN
- 0958-0670 (ISSN-L)
- OpenAlex
- W4238122660
- Cited by
- 313
- References
- 1
- FWCI
- 1.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- Desbaillets, I., Ziegler, U., Groscurth, P., & Gassmann, M. (2000). Embryoid bodies: an in vitro model of mouse embryogenesis. Experimental Physiology. https://doi.org/10.1017/s0958067000021047
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.