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A Microfabricated Fluidic Device for Performing Two-Dimensional Liquid-Phase Separations
Abstract
A microfabricated fluidic device that combines micellar electrokinetic chromatography and high-speed open-channel electrophoresis on a single structure for the rapid automated two-dimensional analysis of peptides has been devised and demonstrated. The microchip operates by rapidly sampling and analyzing effluent in the second dimension from the first dimension. Second-dimension analyses are performed and completed every few seconds, with total analysis times of less than 10 min for tryptic peptides. The peak capacity of the two-dimensional separations has been estimated to be in the 500-1000 range. The orthogonality of the separation techniques, an important factor for maximizing peak capacity or resolution elements, was verified by examining each technique independently for peptide separations. The two-dimensional separation strategy was found to greatly increase the resolving power over that obtained for either dimension alone.
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Cited by
Micro Total Analysis Systems. 2. Analytical Standard Operations and Applications
Auroux, P.-A., Iossifidis, D., Reyes, D. R., & Manz, A. (2002) · Analytical Chemistry · doi:10.1021/ac020239t · Cited by 1,532
Microfluidic chips for clinical and forensic analysis
Verpoorte, E. (2002) · ELECTROPHORESIS · doi:10.1002/1522-2683(200203)23:5<677::aid-elps677>3.0.co;2-8 · Cited by 526
Microfluidic systems in proteomics
Lion, N., Rohner, T. C., Dayon, L., Arnaud, I. L., Damoc, E., Youhnovski, N., Wu, Z., Roussel, C., Josserand, J., Jensen, H., et al. (2003) · ELECTROPHORESIS · doi:10.1002/elps.200305629 · Cited by 262
Capillary electrophoresis applied to proteomic analysis
Fonslow, B. R., & Yates III, J. R. (2009) · Journal of Separation Science · doi:10.1002/jssc.200800592 · Cited by 92
Metadata
- Authors
- Rocklin, R. D., Ramsey, R. S., & Ramsey, J. M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-09-27
- Volume
- 72
- Issue
- 21
- Pages
- 5244-5249
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2011073038
- Cited by
- 196
- References
- 0
- FWCI
- 14.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rocklin, R. D., Ramsey, R. S., & Ramsey, J. M. (2000). A Microfabricated Fluidic Device for Performing Two-Dimensional Liquid-Phase Separations. Analytical Chemistry. https://doi.org/10.1021/ac000578r
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