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LC/MS/MS of carbohydrates with postcolumn addition of metal chlorides using triaxial electrospray probe

Kohler, Martin., Leary, Julie A.·1995·Analytical Chemistry

DOI 10.1021/ac00115a019

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Abstract

A new technique for on-line postcolumn addition (PCA) formation and tandem electrospray mass spectrometry of carbohydrate-metal complexes is presented. A metal chloride solution is added to a carbohydrate sample directly within the ion source of the mass spectrometer. Using a triaxial electrospray probe, this technique can be applied to form carbohydrate-metal complexes on-line, without the need of previously mixing the carbohydrate and metal chloride. Two basic tasks may be accomplished: structural analysis and sensitivity enhancement. The performance of this approach is demonstrated through PCA of LiCl, NaCl, KCl, RbCl, CsCl, and CoCl2, introduced via the triaxial probe after chromatographic separation of two four-component carbohydrate mixtures. Each metal-carbohydrate complex is subsequently analyzed by on-line MS and MS/MS. This technique is used to enhance sensitivity and also, in the case of cobalt coordination, to assist in carbohydrate structural elucidation. On-line LC/MS with PCA of LiCl was achieved with as little as 1.7 pmol of oligosaccharide (average consumed amount, 1.7 pmol with 1 microL of a 10 pmol/microL carbohydrate test mixture injection).

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Authors: Kohler, M., & Leary, J. A.
Affiliations: University of California, Berkeley (US)
Venue: Analytical Chemistry
Publisher: American Chemical Society (ACS)
Published: 1995-10-01
Volume: 67
Issue: 19
Pages: 3501-3508
ISSN: 0003-2700 (ISSN-L)
1520-6882
DOI: 10.1021/ac00115a019
OpenAlex: W2091422516
Cited by: 96
References: 21
Citations by year: 2025: 2 · 2023: 3 · 2022: 3 · 2019: 2 · 2018: 5 · 2016: 1 · 2015: 4 · 2014: 1 · 2013: 3 · 2012: 2
FWCI: 3.62
Type: journal article
Language: en
Open access: closed
Locations: Analytical Chemistry (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute of General Medical Sciences (GM-47356, R01GM047356)
Topics: Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Physical Sciences › Chemistry › Analytical Chemistry › Analytical Chemistry and Chromatography
Keywords: LC-MS/MS, metal chlorides, cobalt complexation, sensitivity enhancement
MeSH terms: Carbohydrates, Chlorides, Chromatography, Liquid, Metals, Mass Spectrometry

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LC/MS/MS of carbohydrates with postcolumn addition of metal chlorides using triaxial electrospray probe

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