The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Charting the Proteomes of Organisms with Unsequenced Genomes by MALDI-Quadrupole Time-of-Flight Mass Spectrometry and BLAST Homology Searching
Abstract
MALDI-quadrupole time-of-flight mass spectrometry was applied to identify proteins from organisms whose genomes are still unknown. The identification was carried out by successively searching a sequence database-first with a peptide mass fingerprint, then with a packet of noninterpreted MS/MS spectra, and finally with peptide sequences obtained by automated interpretation of the MS/MS spectra. A "MS BLAST" homology searching protocol was developed to overcome specific limitations imposed by mass spectrometric data, such as the limited accuracy of de novo sequence predictions. This approach was tested in a small-scale proteomic project involving the identification of 15 bands of gel-separated proteins from the methylotrophic yeast Pichia pastoris, whose genome has not yet been sequenced and which is only distantly related to other fungi.
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Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
The sequence of the human genome
Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) · Science · doi:10.1126/science.1058040 · Cited by 13,909
Cited by
Keller, A., Nesvizhskii, A. I., Kolker, E., & Aebersold, R. (2002) · Analytical Chemistry · doi:10.1021/ac025747h · Cited by 4,830
A Statistical Model for Identifying Proteins by Tandem Mass Spectrometry
Nesvizhskii, A. I., Keller, A., Kolker, E., & Aebersold, R. (2003) · Analytical Chemistry · doi:10.1021/ac0341261 · Cited by 4,809
Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues
Olsen, J. V., Ong, S.-E., & Mann, M. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t400003-mcp200 · Cited by 1,254
A novel MALDI LIFT-TOF/TOF mass spectrometer for proteomics
Suckau, D., Resemann, A., Schuerenberg, M., Hufnagel, P., Franzen, J., & Holle, A. (2003) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-003-2057-0 · Cited by 698
Integrated analysis of shotgun proteomic data with PatternLab for proteomics 4.0
Carvalho, P. C., Lima, D. B., Leprevost, F. V., Santos, M. D. M., Fischer, J. S. G., Aquino, P. F., Moresco, J. J., Yates, J. R., & Barbosa, V. C. (2016) · Nature Protocols · doi:10.1038/nprot.2015.133 · Cited by 283
Metadata
- Authors
- Shevchenko, A., Sunyaev, S., Loboda, A., Shevchenko, A., Bork, P., Ens, W., & Standing, K. G.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-03-23
- Volume
- 73
- Issue
- 9
- Pages
- 1917-1926
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1970172743
- Cited by
- 582
- References
- 3
- FWCI
- 13.73
- Type
- journal article
- Language
- en
- Open access
- closed
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