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Flow Cytometry of Escherichia coli on Microfluidic Devices
Abstract
Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The cells were electrophoretically focused at the channel cross and detected by coincident light scattering and fluorescence. The E. coli were labeled with a membrane-permeable nucleic acid stain (Syto15), a membrane-impermeable nucleic acid stain (propidium iodide), or a fluorescein-labeled antibody and counted at rates from 30 to 85 Hz. The observed labeling efficiencies for the dyes and antibody were greater than 94%.
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Cited by
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Auroux, P.-A., Iossifidis, D., Reyes, D. R., & Manz, A. (2002) · Analytical Chemistry · doi:10.1021/ac020239t · Cited by 1,532
Point-of-Care Diagnostics for Global Health
Yager, P., Domingo, G. J., & Gerdes, J. (2008) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.10.061807.160524 · Cited by 1,076
Abgrall, P., & Gué, A.-M. (2007) · Journal of Micromechanics and Microengineering · doi:10.1088/0960-1317/17/5/r01 · Cited by 561
Lab-on-a-chip for drug development
Weigl, B. H., Bardell, R. L., & Cabrera, C. R. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00223-5 · Cited by 481
Microfluidics technology for manipulation and analysis of biological cells
Yi, C., Li, C.-W., Ji, S., & Yang, M. (2006) · Analytica Chimica Acta · doi:10.1016/j.aca.2005.12.037 · Cited by 478
Microfluidics for flow cytometric analysis of cells and particles
Huh, D., Gu, W., Kamotani, Y., Grotberg, J. B., & Takayama, S. (2005) · Physiological Measurement · doi:10.1088/0967-3334/26/3/r02 · Cited by 396
Microfluidic Devices for the High-Throughput Chemical Analysis of Cells
McClain, M. A., Culbertson, C. T., Jacobson, S. C., Allbritton, N. L., Sims, C. E., & Ramsey, J. M. (2003) · Analytical Chemistry · doi:10.1021/ac0346510 · Cited by 383
Self-citationRecent advances in microfluidic technologies for cell-to-cell interaction studies
Rothbauer, M., Zirath, H., & Ertl, P. (2018) · Lab on a Chip · doi:10.1039/c7lc00815e · Cited by 277
Label-Free Detection of Single Living Bacteria via Electrochemical Collision Event
Lee, J. Y., Kim, B.-K., Kang, M., & Park, J. H. (2016) · Scientific Reports · doi:10.1038/srep30022 · Cited by 89
Metadata
- Authors
- McClain, M. A., Culbertson, C. T., Jacobson, S. C., & Ramsey, J. M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-10-03
- Volume
- 73
- Issue
- 21
- Pages
- 5334-5338
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2080016122
- Cited by
- 224
- References
- 0
- FWCI
- 14.17
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
