Cited by 63Chromatography in Natural ProductsPhytochemical Studies and BioactivitiesAnalytical Chemistry and Chromatography

HPLC Analysis of Ecdysteroids in Plant Extracts Using Superheated Deuterium Oxide with Multiple On-Line Spectroscopic Analysis (UV, IR, 1 H NMR, and MS)

Louden, Dave, Handley, Alan, Lafont, Rene, Taylor, Steve, Sinclair, Ian, Lenz, Eva, Orton, Timothy, Wilson, Ian D.·2002·Analytical Chemistry

Abstract

HPLC, using superheated D20 as the mobile phase, combined with on-line characterization via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and mass spectrometry has been used for the analysis of a standard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometers enabled the on-flow collection of UV, 1H NMR, IR, and mass spectra not only for pure 20-hydroxyecdysone (100-400 microg on column) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.

Integrity signals

Registry

Crossref · 10.1021/ac0107397

Cites

19 of 19 references verified0 citation errors · 0 not checkable

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  • Ecdysteroids can be satisfactorily separated on a C18-RP-HPLC column by superheated (160°C) D2O alone, without an organic modifier.

    “demonstrating that ecdysteroids can be satisfactorily separated on a C 18 -RP-HPLC column by superheated (160 • C) D 2 O alone (i.e., without an organic modifier) [141].”

    From Ecdysteroids (2021) · cites this paper as [141]

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Authors
Louden, D., Handley, A., Lafont, R., Taylor, S., Sinclair, I., Lenz, E., Orton, T., & Wilson, I. D.
Affiliations
  • LGC (GB)
  • Centre National de la Recherche Scientifique (FR)
  • École Normale Superieure, Départment de Biologie, CNRS EP 119,46 rue d'Ulm, F-75230 Paris, Cedex 05, France
Corresponding author
Wilson, Ian D.
Venue
Analytical Chemistry
Published
2001-12-01
Volume
74
Issue
1
Pages
288-294
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W2040942795
Cited by
63
References
19
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 2 · 2014: 1 · 2013: 1 · 2012: 2
FWCI
6.45
Type
journal article
Language
en
Open access
closed
Locations
  • Analytical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
Keywords
ecdysteroids, 20-hydroxyecdysone, 1H NMR, FTIR spectroscopy, mass spectrometry, UV detection, plant extracts, Silene otites, Silene nutans, polypodine B, mobile phase
MeSH terms
Animals, Chromatography, High Pressure Liquid, Equipment Design, Hot Temperature, Plant Extracts, Spectrum Analysis, Deuterium Oxide, Ecdysteroids

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