HPLC Analysis of Ecdysteroids in Plant Extracts Using Superheated Deuterium Oxide with Multiple On-Line Spectroscopic Analysis (UV, IR, 1 H NMR, and MS)
Abstract
HPLC, using superheated D20 as the mobile phase, combined with on-line characterization via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and mass spectrometry has been used for the analysis of a standard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometers enabled the on-flow collection of UV, 1H NMR, IR, and mass spectra not only for pure 20-hydroxyecdysone (100-400 microg on column) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.
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Ecdysteroids can be satisfactorily separated on a C18-RP-HPLC column by superheated (160°C) D2O alone, without an organic modifier.
“demonstrating that ecdysteroids can be satisfactorily separated on a C 18 -RP-HPLC column by superheated (160 • C) D 2 O alone (i.e., without an organic modifier) [141].”
From Ecdysteroids (2021) · cites this paper as [141]
Cites
Shockcor, J. P., Unger, S. E., Wilson, I. D., et al. (1996) · Analytical Chemistry · doi:10.1021/ac9606463 · Cited by 173 · see citing works
Self-citationHansen, S. H., Jensen, A. G., Cornett, C., et al. (1999) · Analytical Chemistry · doi:10.1021/ac990598x · Cited by 127 · see citing works
Diraison, F., Pachiaudi, C., & Beylot, M. (1997) · Journal of Mass Spectrometry · doi:10.1002/(sici)1096-9888(199701)32:1 · Cited by 123 · see citing works
Superheated Heavy Water as the Eluent for HPLC-NMR and HPLC-NMR-MS of Model Drugs
Smith, R. M., Chienthavorn, O., Wilson, I. D., Wright, B., & Taylor, S. D. (1999) · Analytical Chemistry · doi:10.1021/ac9905470 · Cited by 70 · see citing works
Self-citationPullen, F. S., Swanson, A. G., Newman, M. J., et al. (1995) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.1290091106 · Cited by 62 · see citing works
Albert, K., Braumann, U., Tseng, L., et al. (1994) · Analytical Chemistry · doi:10.1021/ac00091a009 · Cited by 60 · see citing works
Scarfe, G. B., Wright, B., Clayton, E. G., et al. (1998) · Xenobiotica · doi:10.1080/004982598239489 · Cited by 57 · see citing works
Clayton, E. G., Taylor, S., Wright, B., et al. (1998) · Chromatographia · doi:10.1007/bf02466530 · Cited by 55 · see citing works
Self-citationWilson, I. D. (2000) · Journal of Chromatography A · doi:10.1016/s0378-4347(00)00071-2 · Cited by 55 · see citing works
Self-citationWilson, I. D., Morgan, E. D., Lafont, R., Shockcor, J. P., Lindon, J. C., Nicholson, J. K., & Wright, B. (1999) · Chromatographia · doi:10.1007/bf02467609 · Cited by 52 · see citing works
Self-citationScarfe, G. B. (1999) · Xenobiotica · doi:10.1080/004982599238821 · Cited by 46 · see citing works
Louden, D., Handley, A., Taylor, S., Lenz, E. M., Miller, S., Wilson, I. D., Sage, A. B., & Lafont, R. (2001) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)01204-8 · Cited by 45 · see citing works
Self-citationDear, G. J., Ayrton, J., Plumb, R. S., et al. (1998) · Rapid Communications in Mass Spectrometry · doi:10.1002/(sici)1097-0231(19981230)12:24 · Cited by 37 · see citing works
Smith, R. M., Chienthavorn, O., Saha, S., et al. (2000) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)00493-3 · Cited by 36 · see citing works
Smith, R. M., Chienthavorn, O., Wilson, I. D., & Wright, B. (1998) · Analytical Communications · doi:10.1039/a804716b · Cited by 35 · see citing works
Self-citationLudlow, M., Louden, D., Handley, A., et al. (1999) · Journal of Chromatography A · doi:10.1016/s0021-9673(99)00778-5 · Cited by 35 · see citing works
Self-citationLouden, D., Handley, A., Taylor, S., et al. (2000) · Analytical Chemistry · doi:10.1021/ac000204y · Cited by 30 · see citing works
Self-citationLudlow, M., Louden, D., Taylor, A. H. S., et al. (1999) · Analytical Communications · doi:10.1039/a900602h · Cited by 12 · see citing works
Self-citationEnnis, J. N., & Buxton, P. C. (1996) · Analytical Communications · doi:10.1039/ac9963300261 · Cited by 2 · see citing works
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Tistaert, C., Dejaegher, B., & Heyden, Y. V. (2011) · Analytica Chimica Acta · doi:10.1016/j.aca.2011.02.023 · Cited by 341 · see citing works
Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
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- Authors
- Louden, D., Handley, A., Lafont, R., Taylor, S., Sinclair, I., Lenz, E., Orton, T., & Wilson, I. D.
- Affiliations
- LGC (GB)
- Centre National de la Recherche Scientifique (FR)
- École Normale Superieure, Départment de Biologie, CNRS EP 119,46 rue d'Ulm, F-75230 Paris, Cedex 05, France
- Corresponding author
- Wilson, Ian D.
- ORCID
- Wilson, Ian D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-12-01
- Volume
- 74
- Issue
- 1
- Pages
- 288-294
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2040942795
- Cited by
- 63
- References
- 19
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 2 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- ecdysteroids, 20-hydroxyecdysone, 1H NMR, FTIR spectroscopy, mass spectrometry, UV detection, plant extracts, Silene otites, Silene nutans, polypodine B, mobile phase
- MeSH terms
- Animals, Chromatography, High Pressure Liquid, Equipment Design, Hot Temperature, Plant Extracts, Spectrum Analysis, Deuterium Oxide, Ecdysteroids
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