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Development of an Aggregation-Based Immunoassay for Anti-Protein A Using Gold Nanoparticles
Abstract
A unique, sensitive, and highly specific immunoassay system for antibodies using gold nanoparticles has been developed. The assay is based on the aggregation of gold nanoparticles that are coated with protein antigens in the presence of their corresponding antibodies. The aggregation of the gold nanoparticles results in an absorption change at 620 nm that is monitored using an absorption plate reader. To demonstrate the analytical capabilities of the new technique, monodispersed protein A-coated gold particles, averaging 10 nm in diameter, were used to determine the level of anti-protein A in serum samples. The effects of the pH, the temperature, and the concentration of protein A-coated gold nanoparticles on the sensitivity of the assay were investigated using transmission electron microscopy (TEM) and UV/vis absorption spectroscopy. A dynamic range of 2 orders of magnitude and a limit of detection of 1 microg/mL of anti-protein A were observed. The new technique could be used for fast, high-throughput screening of antibodies in clinical diagnostic applications.
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Citations
Cited by
Exploitation of Localized Surface Plasmon Resonance
Hutter, E., & Fendler, J. H. (2004) · Advanced Materials · doi:10.1002/adma.200400271 · Cited by 2,726
Gold nanostructures: engineering their plasmonic properties for biomedical applications
Hu, M., Chen, J., Li, Z.-Y., Au, L., Hartland, G. V., Li, X., Marquez, M., & Xia, Y. (2006) · Chemical Society Reviews · doi:10.1039/b517615h · Cited by 1,756
Effects of nanopolystyrene on the feeding behavior of the blue mussel (Mytilus edulis L.)
Wegner, A., Besseling, E., Foekema, E. M., Kamermans, P., & Koelmans, A. A. (2012) · Environmental Toxicology and Chemistry · doi:10.1002/etc.1984 · Cited by 550
New Generation of Multifunctional Nanoparticles for Cancer Imaging and Therapy
Park, K., Lee, S., Kang, E., Kim, K., Choi, K., & Kwon, I. C. (2009) · Advanced Functional Materials · doi:10.1002/adfm.200801655 · Cited by 432
Nanoparticles for detection and diagnosis
Agasti, S. S., Rana, S., Park, M.-H., Kim, C. K., You, C.-C., & Rotello, V. M. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.11.004 · Cited by 313
Huang, K.-J., Niu, D.-J., Xie, W.-Z., & Wang, W. (2010) · Analytica Chimica Acta · doi:10.1016/j.aca.2009.11.023 · Cited by 261
Metadata
- Authors
- Thanh, N. T. K., & Rosenzweig, Z.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-03-06
- Volume
- 74
- Issue
- 7
- Pages
- 1624-1628
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2062906672
- Cited by
- 512
- References
- 0
- FWCI
- 14.84
- Type
- journal article
- Language
- en
- Open access
- closed
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