The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Microscale Nonreductive Release of O-Linked Glycans for Subsequent Analysis through MALDI Mass Spectrometry and Capillary Electrophoresis
Abstract
A new beta-elimination-based procedure has been devised for a microscale release of O-linked oligosaccharides from glycoproteins. Unlike the conventional Carlson degradation, which leads to formation of alditols, the procedure reported here renders the reducing end intact. Conversion of the liberated oligosaccharides to glycosylamines in ammonia medium is followed by the production of the reducing oligosaccharides through the addition of boric acid. The quantitatively generated oligosaccharides with the reducing end can subsequently be derivatized with a fluorophoric reagent for capillary electrophoresis or, alternatively, analyzed through MALDI mass spectrometry. The microscale version of these chemical steps permits us to investigate structurally O-linked oligosaccharides at very low levels.
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Smoothing and Differentiation of Data by Simplified Least Squares Procedures.
Savitzky, A., & Golay, M. J. E. (1964) · Analytical Chemistry · doi:10.1021/ac60214a047 · Cited by 21,904
Cited by
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Self-citationAnumula, K. R. (2006) · Analytical Biochemistry · doi:10.1016/j.ab.2005.09.037 · Cited by 254
Mucin‐type O‐glycosylation – putting the pieces together
Jensen, P. H., Kolarich, D., & Packer, N. H. (2010) · The FEBS Journal · doi:10.1111/j.1742-4658.2009.07429.x · Cited by 252
Analysis of glycans derived from glycoconjugates by capillary electrophoresis‐mass spectrometry
Mechref, Y. (2011) · ELECTROPHORESIS · doi:10.1002/elps.201100342 · Cited by 64
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Metadata
- Authors
- Huang, Y., Mechref, Y., & Novotny, M. V.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-11-16
- Volume
- 73
- Issue
- 24
- Pages
- 6063-6069
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2002613342
- Cited by
- 211
- References
- 1
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- closed
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