The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards
Abstract
A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization. This biotechnology provides differential expression measurements and enables the discovery of biological markers (biomarkers). Work presented here uses human serum but is applicable to any fluid or tissue. The approach relies on linearity of signal versus molecular concentration and reproducibility of sample processing. There is no use of isotopic labeling or chemically similar standard materials. Linear standard curves are reported for a variety of compounds introduced into human serum. As a measure of analytical reproducibility for proteome and metabolome sampling, median coefficients of variation of 25.7 and 23.8%, respectively, were determined for approximately 3400 molecular ions (not counting their numerous isotopes) from 25 independently processed human serum samples, corresponding to a total of 85000 individual molecular ion measurements.
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Cites
Smoothing and Differentiation of Data by Simplified Least Squares Procedures.
Savitzky, A., & Golay, M. J. E. (1964) · Analytical Chemistry · doi:10.1021/ac60214a047 · Cited by 21,904
Dynamic Programming Algorithm Optimization for Spoken Word Recognition
Sakoe, H., & Chiba, S. (1978) · IEEE Transactions on Acoustics Speech and Signal Processing · doi:10.1109/tassp.1978.1163055 · Cited by 6,727
Cited by
Protein biomarker discovery and validation: the long and uncertain path to clinical utility
Rifai, N., Gillette, M. A., & Carr, S. A. (2006) · Nature Biotechnology · doi:10.1038/nbt1235 · Cited by 1,867
Absolute Quantification of Proteins by LCMSE
Silva, J. C., Gorenstein, M. V., Li, G.-Z., Vissers, J. P. C., & Geromanos, S. J. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500230-mcp200 · Cited by 1,529
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Peterson, A. C., Russell, J. D., Bailey, D. J., Westphall, M. S., & Coon, J. J. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.o112.020131 · Cited by 1,288
Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics
Old, W. M., Meyer-Arendt, K., Aveline-Wolf, L., Pierce, K. G., Mendoza, A., Sevinsky, J. R., Resing, K. A., & Ahn, N. G. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500084-mcp200 · Cited by 1,206
Metadata
- Authors
- Wang, W., Zhou, H., Lin, H., Roy, S., Shaler, T. A., Hill, L. R., Norton, S., Kumar, P., Anderle, M., & Becker, C. H.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-08-08
- Volume
- 75
- Issue
- 18
- Pages
- 4818-4826
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2152180036
- Cited by
- 653
- References
- 2
- FWCI
- 14.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wang, W., Zhou, H., Lin, H., Roy, S., Shaler, T. A., Hill, L. R., Norton, S., Kumar, P., Anderle, M., & Becker, C. H. (2003). Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards. Analytical Chemistry. https://doi.org/10.1021/ac026468x
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