The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Raman Detection of Proteomic Analytes
Abstract
The compatibility of nonenhanced Raman spectroscopy with chromatographic and mass spectroscopic proteomic sensing is demonstrated for the first time. High-quality normal Raman spectra are derived from protein solutions with concentrations down to 1 microM and 1 fmol of protein nondestructively probed within the excitation laser beam. These results are obtained using a drop coating deposition Raman (DCDR) method in which the solution of interest is microdeposited (or microprinted) on a compatible substrate, followed by solvent evaporation and backscattering detection. Representative applications include the DCDR detection of insulin derived from an HPLC fraction, nondestructive DCDR followed by MALDI-TOF of lysozyme, the DCDR detection of protein spots deposited using an ink-jet microprinter, and the identification of spectral differences between glycan isomers of equal mass (such as those derived from posttranslationally modified proteins).
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Citations
Cites
Fluidity of Bound Hydration Layers
Raviv, U., & Klein, J. (2002) · Science · doi:10.1126/science.1074481 · Cited by 721
Cited by
Marangoni Effect Reverses Coffee-Ring Depositions
Hu, H., & Larson, R. G. (2006) · The Journal of Physical Chemistry B · doi:10.1021/jp0609232 · Cited by 1,645
Metadata
- Authors
- Zhang, D., Xie, Y., Mrozek, M. F., Ortiz, C., Davisson, V. J., & Ben-Amotz, D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-09-20
- Volume
- 75
- Issue
- 21
- Pages
- 5703-5709
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1964759356
- Cited by
- 188
- References
- 1
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
