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Glucose-Sensitive Holographic Sensors for Monitoring Bacterial Growth

Lee, Mei-Ching, Kabilan, Satyamoorthy, Hussain, Abid, Yang, Xiaoping, Blyth, Jeff, Lowe, Christopher R.·2004·Analytical Chemistry

Abstract

A glucose sensor comprising a reflection hologram incorporated into a thin, acrylamide hydrogel film bearing the cis-diol binding ligand, 3-acrylamidophenylboronic acid (3-APB), is described. The diffraction wavelength (color) of the hologram changes as the polymer swells upon binding cis-diols. The effect of various concentrations of glucose, a variety of mono- and disaccharides, and the alpha-hydroxy acid, lactate, on the holographic response was investigated. The sensor displayed reversible changes in diffraction wavelength as a function of cis-diol concentration, with the sensitivity of the system being dependent on the cis-diol tested. The effect of varying 3-APB concentration in the hydrogel on the holographic response to glucose was investigated, and maximum sensitivity was observed at a functional monomer concentration of 20 mol %. The potential for using this holographic sensor to detect real-time changes in bacterial cell metabolism was demonstrated by monitoring the germination and subsequent vegetative growth of Bacillus subtilis spores.

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Metadata

Authors
Lee, M.-C., Kabilan, S., Hussain, A., Yang, X., Blyth, J., & Lowe, C. R.
Venue
Analytical Chemistry
Published
2004-09-03
Volume
76
Issue
19
Pages
5748-5755
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W1983253767
Cited by
167
References
0
FWCI
2.65
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Glucose-Sensitive Holographic Sensors for Monitoring Bacterial Growth. https://app.cochat.ai/papers/10.1021/ac049334n
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Lee, M.-C., Kabilan, S., Hussain, A., Yang, X., Blyth, J., & Lowe, C. R. (2004). Glucose-Sensitive Holographic Sensors for Monitoring Bacterial Growth. Analytical Chemistry. https://doi.org/10.1021/ac049334n

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