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Glucose-Sensitive Holographic Sensors for Monitoring Bacterial Growth
Abstract
A glucose sensor comprising a reflection hologram incorporated into a thin, acrylamide hydrogel film bearing the cis-diol binding ligand, 3-acrylamidophenylboronic acid (3-APB), is described. The diffraction wavelength (color) of the hologram changes as the polymer swells upon binding cis-diols. The effect of various concentrations of glucose, a variety of mono- and disaccharides, and the alpha-hydroxy acid, lactate, on the holographic response was investigated. The sensor displayed reversible changes in diffraction wavelength as a function of cis-diol concentration, with the sensitivity of the system being dependent on the cis-diol tested. The effect of varying 3-APB concentration in the hydrogel on the holographic response to glucose was investigated, and maximum sensitivity was observed at a functional monomer concentration of 20 mol %. The potential for using this holographic sensor to detect real-time changes in bacterial cell metabolism was demonstrated by monitoring the germination and subsequent vegetative growth of Bacillus subtilis spores.
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Citations
Cited by
Bio-inspired variable structural color materials
Zhao, Y., Xie, Z., Gu, H., Zhu, C., & Gu, Z. (2012) · Chemical Society Reviews · doi:10.1039/c2cs15267c · Cited by 935
Glucose Sensing in Supramolecular Chemistry
Sun, X., & James, T. D. (2015) · Chemical Reviews · doi:10.1021/cr500562m · Cited by 386
Wearable Contact Lens Biosensors for Continuous Glucose Monitoring Using Smartphones
Elsherif, M., Hassan, M. U., Yetisen, A. K., & Butt, H. (2018) · ACS Nano · doi:10.1021/acsnano.8b00829 · Cited by 371
Holographic Sensors: Three-Dimensional Analyte-Sensitive Nanostructures and Their Applications
Yetisen, A. K., Naydenova, I., da Cruz Vasconcellos, F., Blyth, J., & Lowe, C. R. (2014) · Chemical Reviews · doi:10.1021/cr500116a · Cited by 224
Self-citationYetisen, A. K., Butt, H., Volpatti, L. R., Pavlichenko, I., Humar, M., Kwok, S. J. J., Koo, H., Kim, K. S., Naydenova, I., Khademhosseini, A., et al. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.10.005 · Cited by 192
Metadata
- Authors
- Lee, M.-C., Kabilan, S., Hussain, A., Yang, X., Blyth, J., & Lowe, C. R.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-09-03
- Volume
- 76
- Issue
- 19
- Pages
- 5748-5755
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1983253767
- Cited by
- 167
- References
- 0
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lee, M.-C., Kabilan, S., Hussain, A., Yang, X., Blyth, J., & Lowe, C. R. (2004). Glucose-Sensitive Holographic Sensors for Monitoring Bacterial Growth. Analytical Chemistry. https://doi.org/10.1021/ac049334n
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