The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Raman Microscopic Analysis of Single Microbial Cells
Abstract
We demonstrate the utility of the Raman confocal microscope to generate a spectral profile from a single microbial cell and the use of this approach to differentiate bacterial species. In general, profiles from different bacterial taxa shared similar peaks, but the relative abundances of these components varied between different species. The use of multivariate methods subsequently allowed taxa discrimination. Further investigations revealed that the single-cell spectra could be used to differentiate between growth phases of a single species, but these differences did not obscure the overall interspecies discrimination. Finally, we tested the efficacy of the method as a means to identify cells responsible for the uptake of a specific substrate. A single strain was grown in media containing incrementally varying ratios of (13)C(6) to (12)C(6) glucose, and it was found that (13)C incorporation shifted characteristic peaks to lower wavenumbers. These findings suggest that Raman microscopy has significant potential for studies requiring the taxonomic identity and functioning of single microbial cells to be determined.
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Cites
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RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny
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Self-citationFT-INFRARED AND FT-RAMAN SPECTROSCOPY IN BIOMEDICAL RESEARCH
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Cited by
Liu, Y., He, L., Mustapha, A., Li, H., Hu, Z. Q., & Lin, M. (2009) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2009.04303.x · Cited by 892
Life under extreme energy limitation: a synthesis of laboratory- and field-based investigations
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Raman and CARS microspectroscopy of cells and tissues
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Barcoding bacterial cells: a SERS‐based methodology for pathogen identification
Patel, I. S., Premasiri, W. R., Moir, D. T., & Ziegler, L. D. (2008) · Journal of Raman Spectroscopy · doi:10.1002/jrs.2064 · Cited by 207
Metadata
- Authors
- Huang, W. E., Griffiths, R. I., Thompson, I. P., Bailey, M. J., & Whiteley, A. S.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-06-15
- Volume
- 76
- Issue
- 15
- Pages
- 4452-4458
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1997712011
- Cited by
- 411
- References
- 27
- FWCI
- 7.26
- Type
- journal article
- Language
- en
- Open access
- closed
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- Huang, W. E., Griffiths, R. I., Thompson, I. P., Bailey, M. J., & Whiteley, A. S. (2004). Raman Microscopic Analysis of Single Microbial Cells. Analytical Chemistry. https://doi.org/10.1021/ac049753k
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
