The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Chemical Conversion of Nitrate and Nitrite to Nitrous Oxide for Nitrogen and Oxygen Isotopic Analysis in Freshwater and Seawater
Abstract
We present a novel method for nitrogen and oxygen natural isotopic abundance analysis of nitrate and nitrite of seawater and freshwater at environmental concentrations. The method involves the reduction of nitrate to nitrite using spongy cadmium with further reduction to nitrous oxide using sodium azide in an acetic acid buffer. For separate nitrite analysis, the cadmium reduction step is simply bypassed. Nitrous oxide is purged from the water sample and trapped cryogenically using an automated system with subsequent release into a gas chromatography column. The isolated nitrous oxide is then analyzed on a continuous flow isotope ratio mass spectrometer via an open split. This paper describes the basic protocol and reaction conditions required to obtain reproducible natural abundance level nitrogen and oxygen isotopic ratios from nitrate, nitrite, or both, and the results obtained to support these conclusions. A standard deviation less than 0.2 per thousand for nitrogen and 0.5 per thousand for oxygen was found for nitrate samples ranging in concentration from 40 to 0.5 microM (better for nitrite), with a blank of 2 nmol for 50-mL samples. Nitrogen and oxygen isotopic fractionation and oxygen atom exchange were consistent within each batch of analysis. There was no interference from any seawater matrixes. Only one other method published to date can measure the nitrate oxygen isotopic abundance in seawater and none that do so for nitrite alone in the presence of nitrate. This method may prove to be simpler, faster, and obtain isotopic information for lower concentrations of nitrate and nitrite than other methods.
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Cites
A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Seawater and Freshwater
Sigman, D. M., Casciotti, K. L., Andreani, M., Barford, C., Galanter, M., & Böhlke, J. K. (2001) · Analytical Chemistry · doi:10.1021/ac010088e · Cited by 1,956
Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., & Hilkert, A. (2002) · Analytical Chemistry · doi:10.1021/ac020113w · Cited by 1,595
Silva, S. R., Kendall, C., Wilkison, D. H., Ziegler, A. C., Chang, C. C. Y., & Avanzino, R. J. (2000) · Journal of Hydrology · doi:10.1016/s0022-1694(99)00205-x · Cited by 504
Determination of δ18O and δ15N in Nitrate
Revesz, K. M., Böhlke, J. K., & Yoshinari, T. (1997) · Analytical Chemistry · doi:10.1021/ac9610523 · Cited by 76
SHEARER, G. B., & Kohl, D. H. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)37696-8 · Cited by 55
591. Mechanism of the azide–nitrite reaction. Part II
Stedman, G. (1959) · Journal of the Chemical Society (Resumed) · doi:10.1039/jr9590002949 · Cited by 36
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Revising the nitrogen cycle in the Peruvian oxygen minimum zone
Lam, P., Lavik, G., Jensen, M. M., van de Vossenberg, J., Schmid, M., Woebken, D., Gutiérrez, D., Amann, R., Jetten, M. S. M., & Kuypers, M. M. M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0812444106 · Cited by 744
Torres-Martínez, J. A., Mora, A., Knappett, P. S. K., Ornelas-Soto, N., & Mahlknecht, J. (2020) · Water Research · doi:10.1016/j.watres.2020.115962 · Cited by 382
Oxygen Sensitivity of Anammox and Coupled N-Cycle Processes in Oxygen Minimum Zones
Kalvelage, T., Jensen, M. M., Contreras, S., Revsbech, N. P., Lam, P., Günter, M., LaRoche, J., Lavik, G., & Kuypers, M. M. M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0029299 · Cited by 311
Ammonium and nitrite oxidation at nanomolar oxygen concentrations in oxygen minimum zone waters
Bristow, L. A., Dalsgaard, T., Tiano, L., Mills, D. B., Bertagnolli, A. D., Wright, J. J., Hallam, S. J., Ulloa, O., Canfield, D. E., Revsbech, N. P., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1600359113 · Cited by 287
Zhang, Y., Shi, P., Song, J., & Li, Q. (2018) · Applied Sciences · doi:10.3390/app9010018 · Cited by 121
Unraveling Urban NOx Emission Sources in Polluted Arctic Wintertime Using NO2 Nitrogen Isotopes
Albertin, S., Bekki, S., Savarino, J., Brett, N., Law, K. S., Cesler‐Maloney, M., Flynn, J. H., Guo, F., Barret, B., Caillon, N., et al. (2024) · Journal of Geophysical Research: Atmospheres · doi:10.1029/2024jd041842 · Cited by 9
Metadata
- Authors
- McIlvin, M. R., & Altabet, M. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-08-09
- Volume
- 77
- Issue
- 17
- Pages
- 5589-5595
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2048342577
- Cited by
- 718
- References
- 6
- FWCI
- 16.10
- Type
- journal article
- Language
- en
- Open access
- closed
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