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Electrochemical Interrogation of DNA Monolayers on Gold Surfaces
Abstract
In this report, we systematically investigated DNA immobilization at gold surfaces with electrochemical techniques. Comparative cyclic voltammetric and chronocoulometric studies suggested that DNA monolayers immobilized at gold surfaces were not homogeneous. Nonspecific Au-DNA interactions existed even with the treatment of mercaptohexanol, which was known to competitively remove loosely bound DNA at gold surfaces. While both thiolated and nonthiolated DNA formed monolayers on gold surfaces, their hybridization abilities were distinctly different. In contrast to thiolated DNA probes, nonthiolated DNA probes immobilized at gold surfaces were essentially nonhybridizable. The experimental results presented here might be useful for the design of high-performance electrochemical DNA sensors.
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Citations
Cited by
Electrochemistry of Nucleic Acids
Paleček, E., & Bartošík, M. (2012) · Chemical Reviews · doi:10.1021/cr200303p · Cited by 668
A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA
Zhang, J., Song, S., Wang, L., Pan, D., & Fan, C. (2007) · Nature Protocols · doi:10.1038/nprot.2007.419 · Cited by 473
Self-citationHu, K., Lan, D., Li, X., & Zhang, S. (2008) · Analytical Chemistry · doi:10.1021/ac8017197 · Cited by 324
Metadata
- Authors
- Lao, R., Song, S., Wu, H., Wang, L., Zhang, Z., He, L., & Fan, C.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-09-10
- Volume
- 77
- Issue
- 19
- Pages
- 6475-6480
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1966220096
- Cited by
- 250
- References
- 0
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Electrochemical Interrogation of DNA Monolayers on Gold Surfaces. https://app.cochat.ai/papers/10.1021/ac050911x
- The paper
- Lao, R., Song, S., Wu, H., Wang, L., Zhang, Z., He, L., & Fan, C. (2005). Electrochemical Interrogation of DNA Monolayers on Gold Surfaces. Analytical Chemistry. https://doi.org/10.1021/ac050911x
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
