A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA
Abstract
We report a protocol for the amplified detection of target DNA by using a chronocoulometric DNA sensor (CDS). Electrochemistry is known to be rapid, sensitive and cost-effective; it thus offers a promising approach for DNA detection. Our CDS protocol is based on a 'sandwich' detection strategy, involving a capture probe DNA immobilized on a gold electrode and a reporter probe DNA loaded on gold nanoparticles (AuNPs). Each probe flanks one of two fragments of the target sequence. A single DNA hybridization event brings AuNPs, along with hundreds of reporter probes, in the proximity of the electrode. We then employ chronocoulometry to interrogate [Ru(NH3)6]3+ electrostatically bound to the captured DNA strands. This AuNP-amplified DNA sensor can selectively detect as low as femtomolar (zeptomoles) concentrations of DNA targets and conveniently analyze a breast cancer-associated BRCA-1 mutant DNA. The time range for the entire protocol is approximately 3 d, whereas the DNA sensing takes less than 2 h to complete.
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After cross-linking Au NPs on the electrode, surface density increased to 4.062 × 10^13 molecules cm−2, higher than conventional surface-immobilized DNA (~6 × 10^12 molecules cm−2).
“After cross-linking Au NPs on the electrode to increase the surface area, the surface density increased to 4.062 × 1013 molecules cm−2, as shown in fig. S4A, which was higher than that reported for conventional surface-immobilized DNA (~6 × 1012 molecules cm−2) (38, 43).”
From Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage (2021) · cites this paper as [43]
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Xu, C., Ma, B., Gao, Z., Dong, X., Zhao, C., & Liu, H. (2021) · Science Advances · doi:10.1126/sciadv.abk0100 · Cited by 79 · see citing works
- Authors
- Zhang, J., Song, S., Wang, L., Pan, D., & Fan, C.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Fan, Chunhai
- Venue
- Nature Protocols
- Published
- 2007-11
- Volume
- 2
- Issue
- 11
- Pages
- 2888-2895
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W1982883071
- Cited by
- 473
- References
- 17
- Citations by year
- 2026: 10 · 2025: 21 · 2024: 20 · 2023: 21 · 2022: 13 · 2021: 19 · 2020: 28 · 2019: 30 · 2018: 23 · 2017: 23 · 2016: 32 · 2015: 32 · 2014: 26 · 2013: 35 · 2012: 33
- FWCI
- 9.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Keywords
- gold nanoparticles, electrochemical DNA detection, sandwich hybridization assay, breast cancer, gold electrode, signal amplification
- MeSH terms
- Chemistry Techniques, Analytical, DNA, DNA Mutational Analysis, Electrodes, Gold, Humans, Hybridization, Genetic, Biosensing Techniques, Genes, BRCA1, Metal Nanoparticles
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