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Loop-Mediated Isothermal Amplification Integrated on Microfluidic Chips for Point-of-Care Quantitative Detection of Pathogens
Abstract
This work shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during LAMP amplification) or via absorbance measured by an optic sensor; we call this system microLAMP (microLAMP). It is capable of analyzing target nucleic acids quantitatively with high sensitivity and specificity. The assay is straightforward in manipulation. It requires a sample volume of 0.4 microL and is complete within 1 h. The sensitivity of the assay is comparable to standard methods, where 10 fg of DNA sample could be detected under isothermal conditions (63 degrees C). A real time quantitative microLAMP assay using absorbance detection is possible by integration of optical fibers within the chip.
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Citations
Cites
The origins and the future of microfluidics
Whitesides, G. M. (2006) · Nature · doi:10.1038/nature05058 · Cited by 9,687
Cited by
Point-of-care diagnostics for infectious diseases: From methods to devices
Wang, C., Liu, M., Wang, Z., Li, S., Deng, Y., & He, N. (2021) · Nano Today · doi:10.1016/j.nantod.2021.101092 · Cited by 622
Becherer, L., Borst, N., Bakheit, M., Frischmann, S., Zengerle, R., & von Stetten, F. (2020) · Analytical Methods · doi:10.1039/c9ay02246e · Cited by 429
Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP)
Zhang, X., Lowe, S. B., & Gooding, J. J. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.05.039 · Cited by 368
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2012) · Analytical Chemistry · doi:10.1021/ac202611x · Cited by 270
Biomarker detection for disease diagnosis using cost-effective microfluidic platforms
Sanjay, S. T., Fu, G., Dou, M., Xu, F., Liu, R., Qi, H., & Li, X. (2015) · The Analyst · doi:10.1039/c5an00780a · Cited by 267
Metadata
- Authors
- Fang, X., Liu, Y., Kong, J., & Jiang, X.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-03-10
- Volume
- 82
- Issue
- 7
- Pages
- 3002-3006
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2021031313
- Cited by
- 292
- References
- 1
- FWCI
- 15.11
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fang, X., Liu, Y., Kong, J., & Jiang, X. (2010). Loop-Mediated Isothermal Amplification Integrated on Microfluidic Chips for Point-of-Care Quantitative Detection of Pathogens. Analytical Chemistry. https://doi.org/10.1021/ac1000652
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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