The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
High-Performance Isotope Labeling for Profiling Carboxylic Acid-Containing Metabolites in Biofluids by Mass Spectrometry
Abstract
We have developed a new isotope labeling method, based on the use of isotope-coded p-dimethylaminophenacyl (DmPA) bromide as a reagent, combined with liquid chromatography-mass spectrometry (LC-MS) for high-performance metabolome analysis with a focus on profiling carboxylic acid-containing metabolites. Derivatization is simple, fast (1 h plus 30 min for quenching the reaction), and applicable to a wide range of carboxylic acids with a high yield and little or no side reaction products. This labeling method is demonstrated to be not only effective in introducing an isotope tag for accurate metabolite quantification but also improving the chromatographic retention of the metabolites in reversed-phase (RP) LC, enhancing ESI efficiency by 2-4 orders of magnitude, and facilitating the identification of metabolite peaks in LC-MS. In triplicate experiments of a 1:1 ratio of (13)C-/(12)C-DmPA labeled human urine, we were able to detect 2671, 2546, and 2820 ion pairs from metabolites containing one or more carboxylic acid groups.
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Citations
Cites
Human metabolic phenotype diversity and its association with diet and blood pressure
Holmes, E., Loo, R. L., Stamler, J., Bictash, M., Yap, I. K. S., Chan, Q., Ebbels, T., De Iorio, M., Brown, I. J., Veselkov, K. A., et al. (2008) · Nature · doi:10.1038/nature06882 · Cited by 1,054
Scalbert, A., Brennan, L., Fiehn, O., Hankemeier, T., Kristal, B. S., van Ommen, B., Pujos-Guillot, E., Verheij, E., Wishart, D., & Wopereis, S. (2009) · Metabolomics · doi:10.1007/s11306-009-0168-0 · Cited by 565
Cited by
Zhou, B., Xiao, J. F., Tuli, L., & Ressom, H. W. (2011) · Molecular BioSystems · doi:10.1039/c1mb05350g · Cited by 639
Han, J., Lin, K., Sequeira, C., & Borchers, C. H. (2015) · Analytica Chimica Acta · doi:10.1016/j.aca.2014.11.015 · Cited by 521
Mass spectrometric based approaches in urine metabolomics and biomarker discovery
Khamis, M. M., Adamko, D. J., & El‐Aneed, A. (2017) · Mass Spectrometry Reviews · doi:10.1002/mas.21455 · Cited by 342
MyCompoundID: using an evidence-based metabolome library for metabolite identification
Li, L., Li, R., Zhou, J., Zuniga, A., Stanislaus, A. E., Wu, Y., Huan, T., Zheng, J., Shi, Y., Wishart, D. S., et al. (2013) · Analytical Chemistry · doi:10.1021/ac400099b · Cited by 229
Self-citationGuo, B., Chen, B., Liu, A., Zhu, W., & Yao, S. (2012) · Current Drug Metabolism · doi:10.2174/138920012803341401 · Cited by 80
Metadata
- Authors
- Guo, K., & Li, L.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-10-14
- Volume
- 82
- Issue
- 21
- Pages
- 8789-8793
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2335445078
- Cited by
- 193
- References
- 2
- FWCI
- 3.66
- Type
- journal article
- Language
- en
- Open access
- closed
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