The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Horseradish Peroxidase Functionalized Fluorescent Gold Nanoclusters for Hydrogen Peroxide Sensing
Abstract
The fluorescence of metal nanoclusters provides an amusing optic feature to be applied in various fields. However, rational design of dual functional fluorescent metal nanoclusters directed by active enzyme with targeted application remains little explored. In this work, we report a new strategy to construct enzyme functionalized fluorescent gold nanoclusters via a biomineralization process for the detection of hydrogen peroxide. Horseradish peroxidase (HRP) was used as a model functional template to direct the synthesis of fluorescent gold nanoclusters (Au NCs) at physiological conditions to form HRP-Au NCs bioconjugates. We found that the fluorescence of HRP-Au NCs can be quenched quantitatively by adding H(2)O(2), indicating that HRP enzyme remains active and enables catalytic reaction of HRP-Au NCs and H(2)O(2). Upon the addition of H(2)O(2) under optimal conditions, the fluorescence intensity quenched linearly over the range of 100 nM to 100 μM with high sensitivity (LOD = 30 nM, S/N = 3). This study would be potentially extended to other functional proteins to generate dual functional nanoclusters and applied to real time monitoring of biologically important targets in living cells.
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Cites
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Shang, L., Dong, S., & Nienhaus, G. U. (2011) · Nano Today · doi:10.1016/j.nantod.2011.06.004 · Cited by 1,502
Gold Nanomaterials at Work in Biomedicine
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Fluorescent Gold Nanoclusters: Recent Advances in Sensing and Imaging
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Self-citationEngineering ultrasmall water-soluble gold and silver nanoclusters for biomedical applications
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Luminescent Noble Metal Nanoclusters as an Emerging Optical Probe for Sensor Development
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Use of Fluorescent DNA-Templated Gold/Silver Nanoclusters for the Detection of Sulfide Ions
Chen, W.-Y., Lan, G.-Y., & Chang, H.-T. (2011) · Analytical Chemistry · doi:10.1021/ac202162u · Cited by 283
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Organs-on-chip monitoring: sensors and other strategies
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Metadata
- Authors
- Wen, F., Dong, Y., Feng, L., Wang, S., Zhang, S., & Zhang, X.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-01-24
- Volume
- 83
- Issue
- 4
- Pages
- 1193-1196
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2012425908
- Cited by
- 549
- References
- 13
- FWCI
- 23.70
- Type
- journal article
- Language
- en
- Open access
- closed
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