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Cited by 121

The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

Fhaner, Cassie J., Liu, Sichang, Ji, Hong, Simpson, Richard J., Reid, Gavin E.·2012·Analytical Chemistry
DOI 10.1021/ac302154gPMC PMC3491142
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Abstract

A "shotgun" lipidomics strategy consisting of sequential functional group selective chemical modification reactions coupled with high-resolution/accurate mass spectrometry and "targeted" tandem mass spectrometry (MS/MS) analysis has been developed and applied toward the comprehensive identification, characterization and quantitative analysis of changes in relative abundances of >600 individual glycerophospholipid, glycerolipid, sphingolipid and sterol lipids between a primary colorectal cancer (CRC) cell line, SW480, and its isogenic lymph node metastasized derivative, SW620. Selective chemical derivatization of glycerophosphoethanolamine and glycerophosphoserine lipids using a "fixed charge" sulfonium ion containing, d(6)-S,S'-dimethylthiobutanoylhydroxysuccinimide ester (d(6)-DMBNHS) reagent was used to eliminate the possibility of isobaric mass overlap of these species with the precursor ions of all other lipids in the crude extracts, thereby enabling their unambiguous assignment, while subsequent selective mild acid hydrolysis of plasmenyl (vinyl-ether) containing lipids using formic acid enabled these species to be readily differentiated from isobaric mass plasmanyl (alkyl-ether) containing lipids. Using this approach, statistically significant differences in the abundances of numerous lipid species previously identified as being associated with cancer progression or that play known roles as mediators in a range of physiological and pathological processes were observed between the SW480 and SW620 cells. Most notably, these included increased plasmanylcholine and triglyceride lipid levels, decreased plasmenylethanolamine lipids, decreased C-16 containing sphingomyelin and ceramide lipid levels, and a dramatic increase in the abundances of total cholesterol ester and triglyceride lipids in the SW620 cells compared to those in the SW480 cells.

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Research works printed but not linked: 5

  1. Katz-Brull R., 2002, Cancer Res., 62, 1966
  2. Siegel R., 2011, Colorectal Cancer Facts & Figures 2011–2013
  3. Snyder F., 1969, Cancer Res., 29, 251
  4. Howard B. V., 1972, Cancer Res., 32, 1533
  5. Friedberg S. J., 1986, Cancer Res., 46, 845

Cited by

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Metadata

Authors
Fhaner, C. J., Liu, S., Ji, H., Simpson, R. J., & Reid, G. E.
Affiliations
  • Department of Chemistry, Michigan State University, 578 South Shaw Lane, East Lansing, Michigan 48824, United States
  • La Trobe Institute for Molecular Science, La Trobe University, Bundoora Victoria 3086, Australia
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2012-10-10
Volume
84
Issue
21
Pages
8917-8926
ISSN
  • 0003-2700
  • 1520-6882
DOI
10.1021/ac302154g
PMC
PMC3491142
Cited by
121
References
47
Language
en

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