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In Situ Determination of Colloidal Gold Concentrations with UV–Vis Spectroscopy: Limitations and Perspectives
Abstract
This paper studies the UV-vis absorbance of colloidal gold nanoparticles at 400 nm and validates it as a method to determine Au(0) concentrations in colloidal gold solutions. The method is shown to be valid with restrictions depending on the investigated system. The uncertainty of the determined Au(0) concentration can be up to 30%. This deviation is the result of the combined influence of parameters such as particle size, surface modification, or oxidation state. However, quantifying the influence of these parameters enables a much more precise Au(0) determination for specific systems. As an example, the reduction process of the well-known Turkevich method was monitored and the Au(0) concentration was determined with a deviation of less than 5%. Hence, a simple, fast, easy, and cheap in situ method for Au(0) determination is demonstrated that has in the presence of other gold species such as Au(III) an unprecedented accuracy.
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“Hence, the absorbance of the dispersed NPs in the liquid can be linearly correlated to its concentration after a calibration of the spectrophotometer for the given material and solvent.”
From Green nanoparticle synthesis at scale: a perspective on overcoming the limits of pulsed laser ablation in liquids for high-throughput production (2023) · cites this paper as [78]
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Metadata
- Authors
- Hendel, T., Wuithschick, M., Kettemann, F., Birnbaum, A., Rademann, K., & Polte, J.
- Affiliations
- Humboldt-Universität zu Berlin (DE)
- Corresponding author
- Rademann, Klaus
- Polte, Jörg
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-10-29
- Volume
- 86
- Issue
- 22
- Pages
- 11115-11124
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2312797919
- Cited by
- 235
- References
- 97
- Citations by year
- 2026: 15 · 2025: 26 · 2024: 30 · 2023: 30 · 2022: 27 · 2021: 21 · 2020: 22 · 2019: 19 · 2018: 21 · 2017: 5 · 2016: 15 · 2015: 4
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Verband der Chemischen Industrie
- Deutsche Forschungsgemeinschaft (PO 1744/1-1)
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Physical Sciences › Materials Science › Materials Chemistry › Laser-Ablation Synthesis of Nanoparticles
- Physical Sciences › Materials Science › Materials Chemistry › Nanocluster Synthesis and Applications
- Keywords
- colloidal gold nanoparticles, UV-Vis absorbance, in situ monitoring, Turkevich method, nanoparticle reduction, particle size effects, surface modification, oxidation state, measurement uncertainty
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