The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
Abstract
The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse transcription, and PCR amplification with real-time fluorescence detection in the trapped droplets has been developed. The system utilized a shearing T-junction in a fused-silica device to generate a stream of monodisperse picoliter-scale droplets that were isolated from the microfluidic channel walls and each other by the oil-phase carrier. An off-chip valving system stopped the droplets on-chip, allowing thermal cycling for reverse transcription and subsequent PCR amplification without droplet motion. This combination of the established real-time reverse transcription-PCR assay with digital microfluidics is ideal for isolating single-copy RNA and virions from a complex environment and will be useful in viral discovery and gene-profiling applications.
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Vogelstein, B., & Kinzler, K. W. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.16.9236 · Cited by 1,770
Cited by
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets.
Macosko, E. Z., Basu, A., Satija, R., Nemesh, J., Shekhar, K., Goldman, M., Tirosh, I., Bialas, A. R., Kamitaki, N., Martersteck, E. M., et al. (2015) · Cell · doi:10.1016/j.cell.2015.05.002 · Cited by 7,110
High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
Hindson, B. J., Ness, K. D., Masquelier, D. A., Belgrader, P., Heredia, N. J., Makarewicz, A. J., Bright, I. J., Lucero, M. Y., Hiddessen, A. L., Legler, T. C., et al. (2011) · Analytical Chemistry · doi:10.1021/ac202028g · Cited by 2,792
Self-citationSeemann, R., Brinkmann, M., Pfohl, T., & Herminghaus, S. (2012) · Reports on Progress in Physics · doi:10.1088/0034-4885/75/1/016601 · Cited by 1,049
Huggett, J. F., Foy, C. A., Benes, V., Emslie, K., Garson, J. A., Haynes, R., Hellemans, J., Kubista, M., Mueller, R. D., Nolan, T., et al. (2013) · Clinical Chemistry · doi:10.1373/clinchem.2013.206375 · Cited by 859
Quan, P.-L., Sauzade, M., & Brouzes, E. (2018) · Sensors · doi:10.3390/s18041271 · Cited by 664
Metadata
- Authors
- Beer, N. R., Wheeler, E. K., Lee-Houghton, L., Watkins, N., Nasarabadi, S., Hebert, N., Leung, P., Arnold, D. W., Bailey, C. G., & Colston, B. W.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-02-16
- Volume
- 80
- Issue
- 6
- Pages
- 1854-1858
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2067671640
- Cited by
- 209
- References
- 1
- FWCI
- 15.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- Beer, N. R., Wheeler, E. K., Lee-Houghton, L., Watkins, N., Nasarabadi, S., Hebert, N., Leung, P., Arnold, D. W., Bailey, C. G., & Colston, B. W. (2008). On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets. Analytical Chemistry. https://doi.org/10.1021/ac800048k
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