The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Electrochemiluminescence Immunosensor Based on CdSe Nanocomposites
Abstract
A novel strategy for the enhancement of electrochemiluminescence (ECL) was developed by combining CdSe nanocrystals (NCs), carbon nanotube-chitosan (CNT-CHIT), and 3-aminopropyl-triethoxysilane (APS). A label-free ECL immunosensor for the sensitive detection of human IgG (HIgG) was fabricated. The colloidal solution containing CdSe NCs/CNT-CHIT composite was first covered on the Au electrode surface to form a robust film, which showed high ECL intensity and good biocompatibility. After APS as a cross-linker was covalently conjugated to the CdSe NCs/CNT-CHIT film, the ECL intensity was greatly enhanced. And, an intensity about 20-fold higher than that of the CdSe NCs/CNT-CHIT film was observed. After antibody was bound to the functionalized film via glutaric dialdehyde (GLD), the modified electrode could be used as an ECL immunosensor for the detection of HIgG. The specific immunoreaction between HIgG and antibody resulted in the decrease in ECL intensity. The ECL intensity decreased linearly with HIgG concentration in the range of 0.02-200 ng mL(-1), and the detection limit was 0.001 ng mL(-1). The immunosensor has the advantages of high sensitivity, speed, specificity, and stability and could become a promising technique for protein detection.
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Cites
Chemistry and Properties of Nanocrystals of Different Shapes
Burda, C., Chen, X., Narayanan, R., & El-Sayed, M. A. (2005) · Chemical Reviews · doi:10.1021/cr030063a · Cited by 7,230
Perspectives on the Physical Chemistry of Semiconductor Nanocrystals
Alivisatos, A. P. (1996) · The Journal of Physical Chemistry · doi:10.1021/jp9535506 · Cited by 3,595
Colloidal nanocrystal synthesis and the organic–inorganic interface
Yin, Y., & Alivisatos, A. P. (2005) · Nature · doi:10.1038/nature04165 · Cited by 3,273
Cited by
Xu, S., Liu, Y., Wang, T., & Li, J. (2011) · Analytical Chemistry · doi:10.1021/ac200237j · Cited by 367
Li, L., Liu, K., Yang, G., Wang, C., Zhang, J., & Zhu, J. (2011) · Advanced Functional Materials · doi:10.1002/adfm.201001550 · Cited by 329
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Metadata
- Authors
- Jie, G., Zhang, J., Wang, D., Cheng, C., Chen, H.-Y., & Zhu, J.-J.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-04-25
- Volume
- 80
- Issue
- 11
- Pages
- 4033-4039
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1969035592
- Cited by
- 268
- References
- 3
- FWCI
- 10.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jie, G., Zhang, J., Wang, D., Cheng, C., Chen, H.-Y., & Zhu, J.-J. (2008). Electrochemiluminescence Immunosensor Based on CdSe Nanocomposites. Analytical Chemistry. https://doi.org/10.1021/ac800052g
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