The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Development of Quantitative Cell-Based Enzyme Assays in Microdroplets
Abstract
We describe the development of an enzyme assay inside picoliter microdroplets. The enzyme alkaline phosphatase is expressed in Escherichia coli cells and presented in the periplasm. Droplets act as discrete reactors which retain and localize any reaction product. The catalytic turnover of the substrate is measured in individual droplets by monitoring the fluorescence at several time points within the device and exhibits kinetic behavior similar to that observed in bulk solution. Studies on wild type and a mutant enzyme successfully demonstrated the feasibility of using microfluidic droplets to provide time-resolved kinetic measurements.
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Citations
Cites
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
Nikaido, H. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.593-656.2003 · Cited by 4,316
Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents
Song, H., & Ismagilov, R. F. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0354566 · Cited by 693
Cited by
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Single-cell analysis and sorting using droplet-based microfluidics
Mazutis, L., Gilbert, J., Ung, W. L., Weitz, D. A., Griffiths, A. D., & Heyman, J. A. (2013) · Nature Protocols · doi:10.1038/nprot.2013.046 · Cited by 1,355
Droplet microfluidics: recent developments and future applications
Casadevall i Solvas, X., & deMello, A. (2011) · Chem. Commun. · doi:10.1039/c0cc02474k · Cited by 353
Self-citation
Metadata
- Authors
- Huebner, A., Olguin, L. F., Bratton, D., Whyte, G., Huck, W. T. S., de Mello, A. J., Edel, J. B., Abell, C., & Hollfelder, F.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-04-10
- Volume
- 80
- Issue
- 10
- Pages
- 3890-3896
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2068047791
- Cited by
- 193
- References
- 2
- FWCI
- 16.20
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
