Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 348

The DOI matches its Crossref record.

Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions

Liu, Chi-Wei, Huang, Chih-Ching, Chang, Huan-Tsung·2009·Analytical Chemistry
DOI 10.1021/ac8022185
Open this paper in CoChat — free account

Abstract

We have developed a technique for the highly selective and sensitive detection of Pb(2+) and Hg(2+) using a thrombin-binding aptamer (TBA) probe labeled with the donor carboxyfluorescein (FAM) and the quencher 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) at its 5' and 3' termini, respectively. The TBA has a random coil structure that changes into a G-quartet structure and a hairpin-like structure upon binding Pb(2+) and Hg(2+) ions, respectively. As a result, the fluorescence decreases through fluorescence resonance energy transfer (FRET) between the fluorophore and quencher. These changes in fluorescence intensity allow the selective detection of Pb(2+) and Hg(2+) ions at concentrations as low as 300 pM and 5.0 nM using this TBA probe in the presence of phytic acid and a random DNA/NaCN mixture, respectively. The linear correlation existed between the fluorescence intensity and the concentration of Pb(2+) and Hg(2+) over the range of 0.5-30 nM (R(2) = 0.98) and 10-200 nM (R(2) = 0.98), respectively. To the best of our knowledge, this is the first example of a single DNA-based sensor that allows the detection of both Hg(2+) and Pb(2+) ions. This simple and cost-effective probe was also applied to separately determine Pb(2+) in soil samples and spiked Hg(2+) in pond samples.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1021/ac8022185

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 32

  1. Campbell L. M., 2003, J. Toxicol. Environ. Health, Part B, 6, 325, 10.1080/10937400306474 · DOI · Paper page
  2. Needleman H., 2004, Annu. Rev. Med., 55, 209, 10.1146/annurev.med.55.091902.103653 · DOI · Paper page
  3. Needleman H. L., 1991, Human Lead Exposure
  4. Hoyle I., 2005, Aquat. Toxicol., 72, 147, 10.1016/j.aquatox.2004.11.015 · DOI
  5. Zalups R. K., 2000, Pharmacol. Rev., 52, 113, 10.1016/S0031-6997(24)01438-8 · DOI · Paper page
  6. Li Y., 2006, J. Anal. At. Spectrom., 21, 94, 10.1039/B511367A · doi:10.1039/b511367a
  7. Deo S., 2000, J. Am. Chem. Soc., 122, 174, 10.1021/ja992238x · DOI
  8. Nolan E. M., 2003, J. Am. Chem. Soc., 125, 14270, 10.1021/ja037995g · doi:10.1021/ja037995g
  9. Yang Y.-K., 2005, J. Am. Chem. Soc., 127, 16760, 10.1021/ja054855t · DOI · Paper page
  10. Li J., 2000, J. Am. Chem. Soc., 122, 10466, 10.1021/ja0021316 · DOI · Paper page
  11. Liu J., 2003, J. Am. Chem. Soc., 125, 6642, 10.1021/ja034775u · DOI · Paper page
  12. Thomas J. M., 2004, Org. Biomol. Chem., 2, 307, 10.1039/b310154a · doi:10.1039/b310154a
  13. Xiao Y., 2007, J. Am. Chem. Soc., 129, 262, 10.1021/ja067278x · doi:10.1021/ja067278x
  14. Ono A., 2004, Angew. Chem., Int. Ed., 43, 4300, 10.1002/anie.200454172 · DOI · Paper page
  15. Chiang C.-K., 2008, Anal. Chem., 80, 3716, 10.1021/ac800142k · DOI · Paper page
  16. Liu C.-W., 2008, Chem. Commun., 2242, 10.1039/b719856f · doi:10.1039/b719856f
  17. Babkina S. S., 2005, Anal. Chem., 77, 5678, 10.1021/ac050727b · doi:10.1021/ac050727b
  18. Kim I.-B., 2006, J. Am. Chem. Soc., 128, 2818, 10.1021/ja058431a · doi:10.1021/ja058431a
  19. Geary C. D., 2005, Anal. Chem., 77, 185, 10.1021/ac048616k · DOI
  20. Huang C.-C., 2007, Chem. Commun., 12, 1215, 10.1039/B615383F · doi:10.1039/b615383f
  21. Huang C.-C., 2006, Anal. Chem., 78, 8332, 10.1021/ac061487i · DOI · Paper page
  22. Huang C.-C., 2007, Angew. Chem., Int. Ed., 46, 6824, 10.1002/anie.200700803 · DOI · Paper page
  23. Lee J.-S., 2007, Angew. Chem., Int. Ed., 46, 4093, 10.1002/anie.200700269 · DOI · Paper page
  24. Kotch F. W., 2000, Org. Lett., 2, 3277, 10.1021/ol0065120 · doi:10.1021/ol0065120
  25. Smirnov I., 2000, J. Mol. Biol., 296, 1, 10.1006/jmbi.1999.3441 · DOI · Paper page
  26. Smirnov I. V., 2002, Biochemistry, 41, 12133, 10.1021/bi020310p · DOI
  27. Katz S., 1952, J. Am. Chem. Soc., 74, 2238, 10.1021/ja01129a023 · doi:10.1021/ja01129a023
  28. Yamane T., 1961, J. Am. Chem. Soc., 83, 2599, 10.1021/ja01473a001 · doi:10.1021/ja01473a001
  29. Miyake Y., 2006, J. Am. Chem. Soc., 128, 2172, 10.1021/ja056354d · DOI
  30. Tanaka Y., 2007, J. Am. Chem. Soc., 129, 244, 10.1021/ja065552h · DOI · Paper page
  31. 1996, Test Methods for Evaluating Solid Waste, Physical/Chemical Methods
  32. Morel F. M. M., 1983, Principles of Aquatic Chemistry

Cited by

See all 348

Metadata

Authors
Liu, C.-W., Huang, C.-C., & Chang, H.-T.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2009-02-16
Volume
81
Issue
6
Pages
2383-2387
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/ac8022185
OpenAlex
W2015030896
Cited by
348
References
0
FWCI
13.68
Type
journal article
Language
en
Open access
closed

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions. https://app.cochat.ai/papers/10.1021/ac8022185
The paper
Liu, C.-W., Huang, C.-C., & Chang, H.-T. (2009). Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions. Analytical Chemistry. https://doi.org/10.1021/ac8022185

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.