The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Quantitative Microplate-Based Respirometry with Correction for Oxygen Diffusion
Open accessAbstract
Respirometry using modified cell culture microplates offers an increase in throughput and a decrease in biological material required for each assay. Plate based respirometers are susceptible to a range of diffusion phenomena; as O(2) is consumed by the specimen, atmospheric O(2) leaks into the measurement volume. Oxygen also dissolves in and diffuses passively through the polystyrene commonly used as a microplate material. Consequently the walls of such respirometer chambers are not just permeable to O(2) but also store substantial amounts of gas. O(2) flux between the walls and the measurement volume biases the measured oxygen consumption rate depending on the actual [O(2)] gradient. We describe a compartment model-based correction algorithm to deconvolute the biological oxygen consumption rate from the measured [O(2)]. We optimize the algorithm to work with the Seahorse XF24 extracellular flux analyzer. The correction algorithm is biologically validated using mouse cortical synaptosomes and liver mitochondria attached to XF24 V7 cell culture microplates, and by comparison to classical Clark electrode oxygraph measurements. The algorithm increases the useful range of oxygen consumption rates, the temporal resolution, and durations of measurements. The algorithm is presented in a general format and is therefore applicable to other respirometer systems.
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Savitzky-Golay Smoothing Filters
Press, W. H., & Teukolsky, S. A. (1990) · Computers in Physics · doi:10.1063/1.4822961 · Cited by 11,670
Wu, M., Neilson, A., Swift, A. L., Moran, R., Tamagnine, J., Parslow, D., Armistead, S., Lemire, K., Orrell, J., Teich, J., et al. (2007) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00247.2006 · Cited by 918
Self-citationA rapid method for isolation of synaptosomes on Percoll gradients
Dunkley, P. R., Jarvie, P. E., Heath, J. W., Kidd, G. J., & Rostas, J. A. P. (1986) · Brain Research · doi:10.1016/0006-8993(86)91464-2 · Cited by 418
Scott, I. D., & Nicholls, D. G. (1980) · Biochemical Journal · doi:10.1042/bj1860021 · Cited by 393
Self-citationChoi, S. W., Gerencser, A. A., & Nicholls, D. G. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06055.x · Cited by 203
Self-citationDykens, J. A., Jamieson, J. D., Marroquin, L. D., Nadanaciva, S., Xu, J. J., Dunn, M. C., Smith, A. R., & Will, Y. (2008) · Toxicological Sciences · doi:10.1093/toxsci/kfn056 · Cited by 195
PREPARATION OF MITOCHONDRIA FROM ANIMAL TISSUES AND YEASTS
Chappell, J., & Hansford, R. G. (1972) · Elsevier eBooks · doi:10.1016/b978-0-408-70360-4.50009-2 · Cited by 141
O’Riordan, T. C., Zhdanov, A. V., Ponomarev, G. V., & Papkovsky, D. B. (2007) · Analytical Chemistry · doi:10.1021/ac701770b · Cited by 92
Energy Metabolism in Rat Brain Synaptosomes from Nembutal‐Anesthetized and Nonanesthetized Animals
Rafałowska, U., Erecińska, M., & Wilson, D. F. (1980) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1980.tb11218.x · Cited by 68
Gerencser, A. A., Dóczi, J., Törőcsik, B., Bossy‐Wetzel, E., & Ádám‐Vizi, V. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.118620 · Cited by 64
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Assessing bioenergetic function in response to oxidative stress by metabolic profiling
Dranka, B. P., Benavides, G. A., Diers, A. R., Giordano, S., Zelickson, B. R., Reily, C., Zou, L., Chatham, J. C., Hill, B. G., Zhang, J., et al. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.08.005 · Cited by 436
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A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology
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Metadata
- Authors
- Gerencser, A. A., Neilson, A., Choi, S. W., Edman, U., Yadava, N., Oh, R. J., Ferrick, D. A., Nicholls, D. G., & Brand, M. D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-06-25
- Volume
- 81
- Issue
- 16
- Pages
- 6868-6878
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2032102101
- Cited by
- 310
- References
- 12
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
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