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Cited by 436Mitochondrial Function and PathologyRedox biology and oxidative stressCancer, Hypoxia, and Metabolism

Assessing bioenergetic function in response to oxidative stress by metabolic profiling

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Dranka, Brian P., Benavides, Gloria A., Diers, Anne R., Giordano, Samantha, Zelickson, Blake R., Reily, Colin, Zou, Luyun, Chatham, John C. +4 more·2011·Free Radical Biology and Medicine
DOI 10.1016/j.freeradbiomed.2011.08.005PMC PMC3548422
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Abstract

It is now clear that mitochondria are an important target for oxidative stress in a broad range of pathologies, including cardiovascular disease, diabetes, neurodegeneration, and cancer. Methods for assessing the impact of reactive species on isolated mitochondria are well established but constrained by the need for large amounts of material to prepare intact mitochondria for polarographic measurements. With the availability of high-resolution polarography and fluorescence techniques for the measurement of oxygen concentration in solution, measurements of mitochondrial function in intact cells can be made. Recently, the development of extracellular flux methods to monitor changes in oxygen concentration and pH in cultures of adherent cells in multiple-sample wells simultaneously has greatly enhanced the ability to measure bioenergetic function in response to oxidative stress. Here we describe these methods in detail using representative cell types from renal, cardiovascular, nervous, and tumorigenic model systems while illustrating the application of three protocols to analyze the bioenergetic response of cells to oxidative stress.

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  • The effects of TPP+ compounds are likely to be dependent on the cell type since the bioenergetics of cells show a wide range of responses depending on their number of mitochondria and regulation.

    “The effects of TPP+ compounds is also likely to be dependent on the cell type since the bioenergetics of cells show a wide range of responses depending on their number of mitochondria and regulation [30].”

    From Mitochondrially targeted compounds and their impact on cellular bioenergetics (2013) · cites this paper as [30]

    In Protocol 1’s section on HNE responses in multiple cell types (page unavailable), the paper says the integrated bioenergetic response differs by cell type and reports different responses in myocytes, mesangial cells, and SH-SY5Y cells. That supports the cell-type rationale, but the passage concerns HNE, not TPP+ compounds, and does not attribute the differences to mitochondrial number or regulation. (LLM, conf=0.86)

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Authors
Dranka, B. P., Benavides, G. A., Diers, A. R., Giordano, S., Zelickson, B. R., Reily, C., Zou, L., Chatham, J. C., Hill, B. G., Zhang, J., Landar, A., & Darley-Usmar, V. M.
Affiliations
  • University of Alabama at Birmingham (US)
  • University of Louisville (US)
Corresponding author
Darley-Usmar, Victor M.
ORCID
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
2011-11
Volume
51
Issue
9
Pages
1621-1635
ISSN
0891-5849 (ISSN-L)
DOI
10.1016/j.freeradbiomed.2011.08.005
PMC
PMC3548422
OpenAlex
W2072742323
Cited by
436
References
4
Citations by year
2026: 18 · 2025: 19 · 2024: 31 · 2023: 16 · 2022: 36 · 2021: 25 · 2020: 42 · 2019: 28 · 2018: 43 · 2017: 38 · 2016: 37 · 2015: 29 · 2014: 31 · 2013: 33 · 2012: 9
FWCI
9.02
Type
journal article
Language
en
Open access
Licence
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute on Alcohol Abuse and Alcoholism (R01AA013395)
  • National Heart, Lung, and Blood Institute (R01HL096638, R01HL079364, HL007918, T32HL007918, R01HL101192)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK075865, R21DK075867, R01 DK075867)
  • National Institute of Neurological Disorders and Stroke (R01NS064090)
  • National Institute of Environmental Health Sciences (R01ES010167)
  • National Center for Research Resources (P20RR024489)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Keywords
oxidative stress, mitochondrial bioenergetics, extracellular flux analysis, oxygen consumption, mitochondrial function, reactive oxygen species, kidney cells, cardiovascular cells, neurons, cancer cells
MeSH terms
Animals, Cells, Cultured, Energy Metabolism, Humans, Hydrogen-Ion Concentration, Mitochondria, Oxygen, Oxidative Stress

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