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Cited by 419

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

Amperometric Biosensor for Glutamate Using Prussian Blue-Based “Artificial Peroxidase” as a Transducer for Hydrogen Peroxide

Karyakin, Arkady A., Karyakina, Elena E., Gorton, Lo·2000·Analytical Chemistry

Abstract

The specially deposited Prussian Blue denoted as "artificial peroxidase" was used as a transducer for hydrogen peroxide. The electrocatalyst was stable, highly active, and selective to hydrogen peroxide reduction in the presence of oxygen, which allowed sensing of H2O2 around 0.0 V (Ag/AgCl). Glutamate oxidase was immobilized on the surface of the Prussian Blue-modified electrode in a Nafion layer using a nonaqueous enzymology approach. The calibration range for glutamate in flow injection system was 1 x 10(-7)-1 x 10(-4) M. The lowest concentration of glutamate detected (1 x 10(-7) M) and the highest sensitivity in the linear range of 0.21 A M-1 cm-2 were achieved. The influence of reductants was practically avoided using the low potential of an indicator electrode (0.0 V Ag/AgCl). The attractive performance characteristics of the glutamate biosensor illustrate the advantages of Prussian Blue-based "artificial peroxidase" as transducer for hydrogen peroxide detection.

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Metadata

Authors
Karyakin, A. A., Karyakina, E. E., & Gorton, L.
Venue
Analytical Chemistry
Published
2000-03-07
Volume
72
Issue
7
Pages
1720-1723
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W2038787418
Cited by
419
References
15
FWCI
12.56
Type
journal article
Language
en
Open access
closed

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