Sensor and biosensor preparation, optimisation and applications of Prussian Blue modified electrodes
Abstract
Being one of the most commonly used electrochemical mediators for analytical applications, Prussian Blue has found a wide use in the biosensor field during the last years. Its particular characteristic of catalysing hydrogen peroxide reduction has been applied in the construction of a large number of oxidase enzyme-based biosensors for clinical, environmental and food analysis. By modifying an electrode surface with Prussian Blue, it is in fact possible to easily detect hydrogen peroxide at an applied potential around 0.0 V versus Ag/AgCl, thus making possible coupling with oxidase enzymes while also avoiding or reducing electrochemical interferences. Papers dealing with glucose, lactate, cholesterol and galactose biosensors that are based on the use of Prussian Blue have recently appeared in the most important analytical chemistry journals. Another recent trend is the use of a choline probe based on choline oxidase for pesticide determination to exploit the inhibition of acetylcholinesterase by these compounds. In addition, the use of Prussian Blue in the development of biosensors for food analysis has captured the interest of many research groups and led to improved methods for the detection of glutamate, galactose, alcohol, fructosyl amine, formate, lysine and oxalate. This review will focus on the biosensing aspects of Prussian Blue-based sensors giving a general overview of the advantages provided by such mediator as well as its drawbacks. A comprehensive bibliographic reference list is presented together with the most up to date research findings in this field and possible future applications. The commercial potential of sensors based on this mediator will also be discussed.
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The AOx-coated screen-printed PB transducer offers specific low-potential electrocatalytic detection of the hydrogen peroxide product of the AOx enzymatic reaction.
“The amperometric biosensing of alcohol in the generated sweat relied on an AOx-coated screen-printed PB transducer that offers specific low-potential electrocatalytic detection of the hydrogen peroxide product of the AOx enzymatic reaction.33”
From Noninvasive Alcohol Monitoring Using a Wearable Tattoo-Based Iontophoretic-Biosensing System (2016) · cites this paper as [33]
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Self-citation
- Authors
- Ricci, F., & Palleschi, G.
- Affiliations
- University of Rome Tor Vergata (IT)
- Corresponding author
- Ricci, F.
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2005-09
- Volume
- 21
- Issue
- 3
- Pages
- 389-407
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W2144262929
- Cited by
- 781
- References
- 144
- Citations by year
- 2026: 18 · 2025: 23 · 2024: 15 · 2023: 22 · 2022: 34 · 2021: 30 · 2020: 36 · 2019: 41 · 2018: 36 · 2017: 49 · 2016: 51 · 2015: 38 · 2014: 40 · 2013: 37 · 2012: 60
- FWCI
- 2.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Cineca Institutional Research Information System (Tor Vergata University) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- Prussian blue, electrochemical mediator, hydrogen peroxide reduction, electrode modification, glucose biosensor, lactate biosensor, cholesterol biosensor, choline oxidase, acetylcholinesterase inhibition, pesticide detection, food analysis
- MeSH terms
- Electrochemistry, Electrodes, Equipment Design, Ferrocyanides, Oxidoreductases, Transducers, Biosensing Techniques, Equipment Failure Analysis, Coated Materials, Biocompatible
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