In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor
Abstract
Hydrogen peroxide (H 2 O 2 ) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H 2 O 2 assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H 2 O 2 released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H 2 O 2 -responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H 2 O 2 by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H 2 O 2 detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H 2 O 2 detection. Furthermore, the developed SERS sensor enables real-time detection of H 2 O 2 secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal–organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H 2 O 2 secretion, thus exhibiting promising potential for future therapies.
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2-MHQ immobilized on AuNRs reacts with H2O2, oxidizing its hydroxyl group to a carbonyl group to form 2-mercaptobenzoquinone (2-MBQ), causing SERS spectral changes.
“The reaction mechanism of 2-MHQ with H2O2 is shown in Figure 2 A, where the hydroxyl group ( ─ OH) of 2-MHQ is oxidized into carbonyl group ( ═ O), resulting in the formation of 2-mercaptobenzoquinone (2-MBQ) and the changes of SERS spectra when the oxidant H2O2 exists. [30,31]”
From Multiplex Cellular Microenvironment Detection Based on Surface-Enhanced Raman Scattering Patch (2025) · cites this paper as [31]
The MCR two-component model resolved pure component spectra similar to the actual measuring results of pure 2-MHQ and 2-MBQ in the literature, and their contributions vary with H2O2 concentration.
“The resolved pure component spectra (Figure 3B) are similar to the actual measuring results of the pure 2-MHQ and 2-MBQ in literature, [31] and corresponding contributions of pure components (blue or gray curve) vary according to the H2O2 concentration (Figure 3C).”
From Multiplex Cellular Microenvironment Detection Based on Surface-Enhanced Raman Scattering Patch (2025) · cites this paper as [31]
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References 44 · Cited by 54
- Authors
- Jiang, L., He, C.-H., Chen, H.-Y., Xi, C.-Y., Fodjo, E. K., Zhou, Z.-R., Qian, R.-C., Li, D.-W., & Hafez, M. E.
- Affiliations
- East China University of Science and Technology (CN)
- Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China
- Beni-Suef University (EG)
- Corresponding author
- Qian, Ruo-Can
- Li, Da-Wei
- ORCID
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2021-09-09
- Volume
- 93
- Issue
- 37
- Pages
- 12609-12616
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W3198628201
- Cited by
- 54
- References
- 44
- Citations by year
- 2026: 3 · 2025: 13 · 2024: 16 · 2023: 13 · 2022: 9
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (22176058)
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Physical Sciences › Materials Science › Materials Chemistry › Advanced Nanomaterials in Catalysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- hydrogen peroxide detection, surface-enhanced Raman scattering, ZIF-8, metal-organic frameworks, gold nanoparticles, phorbol myristate acetate stimulation, redox signaling, on-chip cell culture, real-time biosensing
- MeSH terms
- Gold, Hydrogen Peroxide, Spectrum Analysis, Raman, Biosensing Techniques, Zeolites, Metal Nanoparticles
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