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High-Throughput Manipulation of Circulating Tumor Cells Using a Multiple Single-Cell Encapsulation System with a Digital Micromirror Device
Abstract
Circulating tumor cells (CTCs) are potential precursors of metastatic cancer, and genomic information obtained from CTCs have the potential to provide new insights into the biology of cancer metastasis. We previously developed a technique for single-cell manipulation based on the encapsulation of a single cell in a photopolymerized hydrogel that can be used for subsequent genetic analysis. However, this technique has limitations in terms of throughput because light irradiation must be performed on each individual cell from the confocal laser-scanning microscopy. Here, we present a high-throughput cell manipulation technique using a multiple single-cell encapsulation system with a digital micromirror device. This system enables rapid cell imaging within a microcavity array, a microfilter for the recovery of CTCs from blood samples, as well as the simultaneous encapsulation of several CTCs with hydrogels photopolymerized using a multiple light-irradiation system. Furthermore, single-cell labeling using two differently shaped hydrogels was examined to distinguish between NCI-H1975 cells and A549 cells, demonstrating the utility of the system for single-cell gene mutation analysis. In addition to CTCs, our system can be widely applied for analyses of mammalian cells and microorganisms.
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The high throughput microfluidic chip demonstrated that large samples were detected in a short time, which improved the accuracy of rare CTC detection.
“The high throughput (i.e., flow rate) microfluidic chip demonstrated that the large samples were detected in a short time, which improved the accuracy of rare CTC detection.35”
From Dynamic Halbach array magnet integrated microfluidic system for the continuous-flow separation of rare tumor cells (2019) · cites this paper as [35]
Citations
Cites
NIH Image to ImageJ: 25 years of image analysis
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012) · Nature Methods · doi:10.1038/nmeth.2089 · Cited by 64,638
Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer
Cristofanilli, M., Budd, G. T., Ellis, M. J., Stopeck, A., Matera, J., Miller, M. C., Reuben, J. M., Doyle, G. V., Allard, W. J., Terstappen, L. W. M. M., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa040766 · Cited by 4,602
Isolation of rare circulating tumour cells in cancer patients by microchip technology
Nagrath, S., Sequist, L. V. D., Maheswaran, S., Bell, D. W., Irimia, D., Ulkus, L., Smith, M. R., Kwak, E. L., Digumarthy, S. R., Muzikansky, A., et al. (2007) · Nature · doi:10.1038/nature06385 · Cited by 3,595
Continuous liquid interface production of 3D objects
Tumbleston, J. R., Shirvanyants, D. G., Ermoshkin, N., Janusziewicz, R., Johnson, A. R., Kelly, D., Chen, K., PINSCHMIDT, R. K. J., Rolland, J. P., Ermoshkin, A. V., et al. (2015) · Science · doi:10.1126/science.aaa2397 · Cited by 2,295
Cohen, S. J., Punt, C. J. A., Iannotti, N. O., Saidman, B. H., Sabbath, K. D., Gabrail, N. Y., Picus, J., Morse, M., Mitchell, E. P., Miller, M. C., et al. (2008) · Journal of Clinical Oncology · doi:10.1200/jco.2007.15.8923 · Cited by 1,872
Massively parallel manipulation of single cells and microparticles using optical images
Chiou, P. Y., Ohta, A. T., & Wu, M. C. (2005) · Nature · doi:10.1038/nature03831 · Cited by 1,455
Riethdorf, S., Fritsche, H. A., Müller, V., Rau, T., Schindlbeck, C., Rack, B. K., Janni, W., Coith, C., Beck, K., Jänicke, F., et al. (2007) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-06-1695 · Cited by 1,342
Isolation by Size of Epithelial Tumor Cells
Vona, G., Sabile, A., Louha, M., Sitruk, V., Romana, S. P., Schütze, K., Capron, F., Franco, D., Pazzagli, M., Vekemans, M. J. J., et al. (2000) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64706-2 · Cited by 1,068
Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting
Ma, X., Qu, X., Zhu, W. Z., Li, Y.-S. J., Yuan, S., Zhang, H., Liu, J., Wang, P., Lai, C. S. E., Zanella, F., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524510113 · Cited by 881
RNA-Seq of single prostate CTCs implicates noncanonical Wnt signaling in antiandrogen resistance
Miyamoto, D. T., Zheng, Y., Wittner, B. S., Lee, R. J., Zhu, H., Broderick, K. T., Desai, R. N., Fox, D. B., Brannigan, B. W., Trautwein, J., et al. (2015) · Science · doi:10.1126/science.aab0917 · Cited by 716
Circulating Tumor Cell Number and Prognosis in Progressive Castration-Resistant Prostate Cancer
Danila, D. C., Heller, G., Gignac, G. A., Gonzalez-Espinoza, R., Anand, A., Tanaka, E., Lilja, H. G., Schwartz, L. B., Larson, S. M., Fleisher, M., et al. (2007) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-07-1506 · Cited by 665
Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer
Lohr, J. G., Adalsteinsson, V. A., Cibulskis, K., Choudhury, A. D., Rosenberg, M. W., Cruz‐Gordillo, P., Francis, J. M., Zhang, C.-Z., Shalek, A. K., Satija, R., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.2892 · Cited by 542
Light-induced cell damage in live-cell super-resolution microscopy
Wäldchen, S., Lehmann, J., Klein, T., van de Linde, S., & Sauer, M. (2015) · Scientific Reports · doi:10.1038/srep15348 · Cited by 541
Heitzer, E., Auer, M., Gasch, C., Pichler, M., Ulz, P., Hoffmann, E. M., Lax, S., Waldispuehl‐Geigl, J., Mauermann, O., Lackner, C., et al. (2013) · Cancer Research · doi:10.1158/0008-5472.can-12-4140 · Cited by 507
Ni, X., Zhuo, M., Su, Z., Duan, J., Gao, Y., Wang, Z., Zong, C., Bai, H., Chapman, A. R., Zhao, J., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1320659110 · Cited by 454
Size-Selective Microcavity Array for Rapid and Efficient Detection of Circulating Tumor Cells
Hosokawa, M., Hayata, T., Fukuda, Y., Arakaki, A., Yoshino, T., Tanaka, T., & Matsunaga, T. (2010) · Analytical Chemistry · doi:10.1021/ac101222x · Cited by 346
Self-citationMiyamoto, D. T., Lee, R. J., Stott, S. L., Ting, D. T., Wittner, B. S., Ulman, M. J., Smas, M. E., Lord, J. B., Brannigan, B. W., Trautwein, J., et al. (2012) · Cancer Discovery · doi:10.1158/2159-8290.cd-12-0222 · Cited by 272
Raimondi, C., Gradilone, A., Naso, G., Vincenzi, B., Petracca, A., Nicolazzo, C., Palazzo, A., Saltarelli, R., Spremberg, F., Cortesi, E., et al. (2011) · Breast Cancer Research and Treatment · doi:10.1007/s10549-011-1373-x · Cited by 269
Molecular profiling of single circulating tumor cells with diagnostic intention
Polzer, B., Medoro, G., Pasch, S., Fontana, F., Zorzino, L., Pestka, A., Andergassen, U., Meier-Stiegen, F., Czyż, Z. T., Alberter, B., et al. (2014) · EMBO Molecular Medicine · doi:10.15252/emmm.201404033 · Cited by 267
Fabbri, F., Carloni, S., Zoli, W., Ulivi, P., Gallerani, G., Fici, P., Chiadini, E., Passardi, A., Frassineti, G. L., Ragazzini, A., et al. (2013) · Cancer Letters · doi:10.1016/j.canlet.2013.02.015 · Cited by 222
Digital microfabrication of user‐defined 3D microstructures in cell‐laden hydrogels
Soman, P., Chung, P. H., Zhang, A. P., & Chen, S. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.24957 · Cited by 213
Hosokawa, M., Kenmotsu, H., Koh, Y., Yoshino, T., Yoshikawa, T., Naito, T., Takahashi, T., Murakami, H., Nakamura, Y., Tsuya, A., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0067466 · Cited by 180
Self-citationPeeters, D. J. E., Laere, B. D., den Eynden, G. G. V., Laere, S. V., Rothé, F., Ignatiadis, M., Sieuwerts, A. M., Lambrechts, D., Rutten, A., van Dam, P. A., et al. (2013) · British Journal of Cancer · doi:10.1038/bjc.2013.92 · Cited by 179
Pestrin, M., Salvianti, F., Galardi, F., Luca, F. D., Turner, N. H., Malorni, L., Pazzagli, M., Leo, A. D., & Pinzani, P. (2014) · Molecular Oncology · doi:10.1016/j.molonc.2014.12.001 · Cited by 168
Luca, F. D., Rotunno, G., Salvianti, F., Galardi, F., Pestrin, M., Gabellini, S., Simi, L., Mancini, I., Vannucchi, A. M., Pazzagli, M., et al. (2016) · Oncotarget · doi:10.18632/oncotarget.8431 · Cited by 157
Microfluidic enrichment for the single cell analysis of circulating tumor cells
Yeo, T., Tan, S. J., Lim, C. L., Lau, D. P. X., Chua, Y. W., Krisna, S. S., Iyer, G., Tan, G. S., Lim, T. K. H., Tan, D. S. W., et al. (2016) · Scientific Reports · doi:10.1038/srep22076 · Cited by 123
Fernandez, S. V., Bingham, C., Fittipaldi, P., Austin, L., Palazzo, J., Palmer, G. A., Alpaugh, K. R., & Cristofanilli, M. (2014) · Breast Cancer Research · doi:10.1186/s13058-014-0445-3 · Cited by 106
Hosokawa, M., Yoshikawa, T., Negishi, R., Yoshino, T., Koh, Y., Kenmotsu, H., Naito, T., Takahashi, T., Yamamoto, N., Kikuhara, Y., et al. (2013) · Analytical Chemistry · doi:10.1021/ac400167x · Cited by 103
Self-citationPancreatic cancer: Circulating Tumor Cells and Primary Tumors show Heterogeneous KRAS Mutations
Kulemann, B., Rösch, S., Seifert, S., Timme, S., Bronsert, P., Seifert, G. J., Martini, V., Kuvendjiska, J., Glatz, T., Hussung, S., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-04601-z · Cited by 96
Swennenhuis, J. F., Reumers, J., Thys, K., Aerssens, J., & Terstappen, L. W. M. M. (2013) · Genome Medicine · doi:10.1186/gm510 · Cited by 88
Xu, L., Mao, X., Guo, T., Chan, P. Y., Shaw, G. L., Hines, J. E. W., Stankiewicz, E., Wang, Y., Oliver, R. T. D., Ahmad, A. S., et al. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-16-3081 · Cited by 80
Yusa, A., Toneri, M., Masuda, T., Ito, S., Yamamoto, S., Okochi, M., Kondo, N., Iwata, H., Yatabe, Y., Ichinosawa, Y., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0088821 · Cited by 78
Rapid Fabrication of Hydrogel Microstructures Using UV-Induced Projection Printing
Yang, W., Yu, H., Liang, W., Wang, Y., & Liu, L. (2015) · Micromachines · doi:10.3390/mi6121464 · Cited by 70
Kim, J., Cho, H., Han, S., & Han, K.-H. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b00570 · Cited by 70
Direct 3D-printing of cell-laden constructs in microfluidic architectures
Liu, J., Hwang, H. H., Wang, P., Whang, G. J., & Chen, S. (2016) · Lab on a Chip · doi:10.1039/c6lc00144k · Cited by 59
Bingham, C., Fernandez, S. V., Fittipaldi, P., Dempsey, P. W., Ruth, K. J., Cristofanilli, M., & Alpaugh, R. K. (2017) · Breast Cancer Research and Treatment · doi:10.1007/s10549-017-4176-x · Cited by 47
Lampignano, R., Yang, L., Neumann, M., Franken, A., Fehm, T. N., Niederacher, D., & Neubauer, H. J. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18091885 · Cited by 45
Yagi, S., Koh, Y., Akamatsu, H., Kanai, K., Hayata, A., Tokudome, N., Akamatsu, K., Endo, K., Nakamura, S., Higuchi, M., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0179744 · Cited by 44
Yoshino, T., Tanaka, T., Nakamura, S., Negishi, R., Hosokawa, M., & Matsunaga, T. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b01475 · Cited by 33
Self-citationControlling uniformity of photopolymerized microscopic hydrogels
Park, S., Kim, D., Ko, S. Y., Park, J., Akella, S., Xu, B., Zhang, Y., & Fraden, S. (2014) · Lab on a Chip · doi:10.1039/c4lc00158c · Cited by 31
Development of the automated circulating tumor cell recovery system with microcavity array
Negishi, R., Hosokawa, M., Nakamura, S., Kanbara, H., Kanetomo, M., Kikuhara, Y., Tanaka, T., Matsunaga, T., & Yoshino, T. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.09.002 · Cited by 25
Self-citationMaeda, Y., Dobashi, H., Sugiyama, Y., Saeki, T., Lim, T.-K., Harada, M., Matsunaga, T., Yoshino, T., & Tanaka, T. (2017) · PLoS ONE · doi:10.1371/journal.pone.0174723 · Cited by 24
Self-citationYoshino, T., Takai, K., Negishi, R., Saeki, T., Kanbara, H., Kikuhara, Y., Matsunaga, T., & Tanaka, T. (2017) · Analytica Chimica Acta · doi:10.1016/j.aca.2017.03.030 · Cited by 20
Self-citationMonitoring of cellular behaviors by microcavity array-based single-cell patterning
Osada, K., Hosokawa, M., Yoshino, T., & Tanaka, T. (2013) · The Analyst · doi:10.1039/c3an01698f · Cited by 18
Self-citationWong, V. C.-L., Ko, J. M. Y., Lam, C.-T., & Lung, M. L. (2017) · PLoS ONE · doi:10.1371/journal.pone.0177276 · Cited by 15
Swennenhuis, J. F., & Terstappen, L. W. M. M. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2990-0_4 · Cited by 14
Yoon, J., Kim, K., & Park, W. (2017) · Applied Physics Letters · doi:10.1063/1.4986393 · Cited by 14
Imaging multiple biomarkers in captured rare cells by sequential immunostaining and photobleaching
Zhao, M., Wei, B., & Chiu, D. T. (2013) · Methods · doi:10.1016/j.ymeth.2013.08.006 · Cited by 10
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Metadata
- Authors
- Negishi, R., Takai, K., Tanaka, T., Matsunaga, T., & Yoshino, T.
- Affiliations
- Tokyo University of Agriculture and Technology (JP)
- Corresponding author
- Yoshino, Tomoko
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-07-24
- Volume
- 90
- Issue
- 16
- Pages
- 9734-9741
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2884458219
- Cited by
- 24
- References
- 48
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 5 · 2018: 1
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Core Research for Evolutional Science and Technology (JPMJCR14G5)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Innovative Microfluidic and Catalytic Techniques Innovation
- Keywords
- circulating tumor cells, single-cell encapsulation, digital micromirror device, microcavity arrays, microfilters, single-cell labeling, H1975 cells, A549 cells, gene mutation analysis, metastasis
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