Cytosolic Uptake of Large Monofunctionalized Dextrans
Open accessAbstract
Dextrans are a versatile class of polysaccharides with applications that span medicine, cell biology, food science, and consumer goods. Here, we report on a new type of large monofunctionalized dextran that exhibits unusual properties: efficient cytosolic and nuclear uptake. This dextran permeates various human cell types without the use of transfection agents, electroporation, or membrane perturbation. Cellular uptake occurs primarily through active transport via receptor-mediated processes. These monofunctionalized dextrans could serve as intracellular delivery platforms for drugs or other cargos.
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The spherical nanomicelles were produced by self-assembly of amphiphilic copolymer tetraphenylethene (TPE) conjugated-ss-poly(aspartic acid)-block-poly(2-methacryloyloxyethyl phosphorylcholine) (TPE-SS-PLAsp-b-PMPC) and subsequently, DOX was encapsulated in them.
“The spherical nanomicelles were produced by self-assembly of amphiphilic copolymer tetraphenylethene (TPE) conjugated-ss-poly(aspartic acid)-block-poly(2-methacryloyloxyethyl phosphorylcholine) (TPE-SS-PLAsp-b-PMPC) and subsequently, DOX was encapsulated in them.”
From Recent Advances in Nanomicelles Delivery Systems. (2020) · cites this paper as [219]
Split into 2 atoms — 0 confirmed, 0 partially supported, 0 disputed, 0 misattributed, 2 unverifiable. Expand the claim to see per-atom verdicts.
Experimental — accuracy not yet measuredA novel redox-responsive amphiphilic micellar system with a dual function of cancer chemotherapy and bioimaging was developed by Wang's group.
“A novel redox-responsive amphiphilic micellar system with a dual function of cancer chemotherapy and bioimaging was developed by Wang’s group [219].”
From Recent Advances in Nanomicelles Delivery Systems. (2020) · cites this paper as [219]
The Introduction (page unavailable) describes a fluorogenic dextran for monitoring cellular uptake, not a redox-responsive micellar system for chemotherapy and bioimaging. Searches for the claimed system and its functions found no passage in our copy attributing such a development to Wang’s group. (LLM, conf=0.76)
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Cites
Ellman, G. L. (1959) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(59)90090-6 · Cited by 26,824 · see citing works
Koppel, D. E. (1972) · The Journal of Chemical Physics · doi:10.1063/1.1678153 · Cited by 2,883 · see citing works
Thiol-ene “click” reactions and recent applications in polymer and materials synthesis
Lowe, A. B. (2010) · Polym. Chem. · doi:10.1039/b9py00216b · Cited by 1,563 · see citing works
Novel and established applications of microbial polysaccharides
Sutherland, I. W. (1998) · Trends in biotechnology · doi:10.1016/s0167-7799(97)01139-6 · Cited by 678 · see citing works
Rapid acidification of endocytic vesicles containing α2-macroglobulin
Tycko, B., & Maxfield, F. R. (1982) · Cell · doi:10.1016/0092-8674(82)90219-7 · Cited by 673 · see citing works
Koniev, O., & Wagner, A. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00048c · Cited by 600 · see citing works
Yamashiro, D. J., Tycko, B., Fluss, S. R., & Maxfield, F. R. (1984) · Cell · doi:10.1016/0092-8674(84)90414-8 · Cited by 577 · see citing works
Li, Y., Zhu, L., Liu, Z., Cheng, R., Meng, F., Cui, J., Ji, S., & Zhong, Z. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200904260 · Cited by 451 · see citing works
Glycan Microarrays for Decoding the Glycome
Rillahan, C. D., & Paulson, J. C. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-061809-152236 · Cited by 429 · see citing works
Colocalization of fluorescent markers in confocal microscope images of plant cells
French, A. P., Mills, S. J., Swarup, R., Bennett, M. J., & Pridmore, T. (2008) · Nature Protocols · doi:10.1038/nprot.2008.31 · Cited by 369 · see citing works
Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
Hughes, L. D., Rawle, R. J., & Boxer, S. G. (2014) · PLoS ONE · doi:10.1371/journal.pone.0087649 · Cited by 293 · see citing works
Dextran conjugates in drug delivery
Varshosaz, J. (2012) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2012.673580 · Cited by 243 · see citing works
Biopolymers from Lactic Acid Bacteria. Novel Applications in Foods and Beverages
Torino, M. I., Font de Valdez, G., & Mozzi, F. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00834 · Cited by 201 · see citing works
The effect of the size of fluorescent dextran on its endocytic pathway
Li, L., Wan, T., Wan, M., Liu, B., Cheng, R., & Zhang, R. (2015) · Cell Biology International · doi:10.1002/cbin.10424 · Cited by 199 · see citing works
Structure Properties of Dextran. 2. Dilute Solution
Ioan, C. E., Aberle, T., & Burchard, W. (2000) · Macromolecules · doi:10.1021/ma000282n · Cited by 179 · see citing works
The available intravenous iron formulations: History, efficacy, and toxicology
Auerbach, M., & Macdougall, I. C. (2017) · Hemodialysis International · doi:10.1111/hdi.12560 · Cited by 158 · see citing works
Insight on the periodate oxidation of dextran and its structural vicissitudes
Maia, J. M., Carvalho, R. A., Coelho, J. F. J., Simões, P. N., & Gil, M. H. (2010) · Polymer · doi:10.1016/j.polymer.2010.11.058 · Cited by 156 · see citing works
Dextran prodrugs — structure and stability in relation to therapeutic activity
Larsen, C. (1989) · Advanced Drug Delivery Reviews · doi:10.1016/0169-409x(89)90006-9 · Cited by 138 · see citing works
Valkevich, E. M., Guenette, R. G., Sanchez, N. A., Chen, Y., Ge, Y., & Strieter, E. R. (2012) · Journal of the American Chemical Society · doi:10.1021/ja300500a · Cited by 136 · see citing works
Deriy, L. V., Gomez, E. A., Zhang, G., Beacham, D. W., Hopson, J. A., Gallan, A. J., Shevchenko, P. D., Bindokas, V. P., & Nelson, D. J. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.057372 · Cited by 135 · see citing works
A Direct Method for Site‐Specific Protein Acetylation
Li, F., Allahverdi, A., Yang, R., Lua, G. B. J., Zhang, X., Cao, Y., Korolev, N., Nordenskiöld, L., & Liu, C. (2011) · Angewandte Chemie International Edition · doi:10.1002/anie.201103754 · Cited by 133 · see citing works
Periodate Oxidation of Dextran
Jeanes, A., & Wilham, C. A. (1950) · Journal of the American Chemical Society · doi:10.1021/ja01162a086 · Cited by 117 · see citing works
Woodroofe, C. C., Masalha, R., Barnes, K. R., Frederickson, C. J., & Lippard, S. J. (2004) · Chemistry & Biology · doi:10.1016/j.chembiol.2004.09.013 · Cited by 115 · see citing works
Poirier, A., Lavé, T., Portmann, R., Brun, M.-E., Senner, F., Kansy, M., Grimm, H.-P., & Funk, C. (2008) · Drug Metabolism and Disposition · doi:10.1124/dmd.108.020750 · Cited by 100 · see citing works
Nuclear actin and myosin as control elements in nucleocytoplasmic transport.
Schindler, M., & Jiang, L. W. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.102.3.859 · Cited by 95 · see citing works
Analysis of Receptor-Ligand Interactions
Attie, A., & Raines, R. T. (1995) · Journal of Chemical Education · doi:10.1021/ed072p119 · Cited by 93 · see citing works
Self-citation[33] Use of horseradish peroxidase and fluorescent dextrans to study fluid pinocytosis in leukocytes
Oliver, J. M., Berlin, R. D., & Davis, B. H. (1984) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(84)08100-3 · Cited by 52 · see citing works
Temperature dependence of endocytosis in renal epithelial cells in culture
Mamdouh, Z., Giocondi, M., Laprade, R., & Grimellec, C. L. (1996) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(96)00077-6 · Cited by 48 · see citing works
Oliver, S., Thomas, D. S., Kavallaris, M., Vittorio, O., & Boyer, C. A. (2016) · Polymer Chemistry · doi:10.1039/c6py00228e · Cited by 47 · see citing works
A simple quantitative fluorimetric assay of in vitro phagocytosis in human neutrophils
Oben, J. A., & Foreman, J. C. (1988) · Journal of Immunological Methods · doi:10.1016/0022-1759(88)90039-7 · Cited by 42 · see citing works
A fluorescence-based in vitro assay for investigating early endosome dynamics
Barysch, S. V., Jahn, R., & Rizzoli, S. O. (2010) · Nature Protocols · doi:10.1038/nprot.2010.84 · Cited by 39 · see citing works
Temperature dependence of protein transport across lymphatic endothelium in vitro.
O’Morchoe, C. C. C., Jones, W. R., Jarosz, H. M., O’Morchoe, P. J., & Fox, L. M. (1984) · The Journal of Cell Biology · doi:10.1083/jcb.98.2.629 · Cited by 38 · see citing works
Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
Jiang, L. W., & Schindler, M. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.102.3.853 · Cited by 37 · see citing works
Dextran structural details from high-field proton NMR spectroscopy
Cheetham, N. W. H., Fiala‐Beer, E., & Walker, G. J. (1990) · Carbohydrate Polymers · doi:10.1016/0144-8617(90)90027-p · Cited by 37 · see citing works
Selective chemical modifications of dextran
Yalpani, M., & Brooks, D. E. (1985) · Journal of Polymer Science Polymer Chemistry Edition · doi:10.1002/pol.1985.170230513 · Cited by 36 · see citing works
Ca2+-dependent and -independent mechanisms of calmodulin nuclear translocation
Thorogate, R., & Török, K. (2004) · Journal of Cell Science · doi:10.1242/jcs.01510 · Cited by 35 · see citing works
Zhang, D., Lee, H.-F., Pettit, S. C., Zaro, J. L., Huang, N., & Shen, W. (2012) · BMC Biotechnology · doi:10.1186/1472-6750-12-92 · Cited by 26 · see citing works
Electronic and Steric Optimization of Fluorogenic Probes for Biomolecular Imaging
Chyan, W., Kilgore, H. R., Gold, B., & Raines, R. T. (2017) · The Journal of Organic Chemistry · doi:10.1021/acs.joc.7b00285 · Cited by 25 · see citing works
Self-citationNordmeier, E. H., Xing, H., & Lechner, M. D. (1993) · Die Makromolekulare Chemie · doi:10.1002/macp.1993.021941101 · Cited by 17 · see citing works
Dextran functionalization enhances nanoparticle-mediated siRNA delivery and silencing
Vocelle, D. B., Chesniak, O. M., Malefyt, A., Comiskey, G., Adu‐Berchie, K., Smith, M. R., Chan, C., & Walton, S. P. (2016) · TECHNOLOGY · doi:10.1142/s2339547816400100 · Cited by 16 · see citing works
Elastic and quasielastic light scattering studies on the branching characteristics of dextrans
Fahner, E. M., Großmann, G. H., & Ebert, K. H. (1984) · Die Makromolekulare Chemie · doi:10.1002/macp.1984.021851015 · Cited by 15 · see citing works
Koniev, O., & Wagner, A. (2015) · Chemical Society Reviews · doi:10.1039/c5cs90060c · Cited by 14 · see citing works
Magini, A., Polchi, A., Tancini, B., Urbanelli, L., Cristina, M. D., Mannucci, R., Nicoletti, I., & Emiliani, C. (2013) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-397926-1.00002-0 · Cited by 4 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [8]Marsh M 2001 Endocytosis Oxford University Press Oxford, UK
- [13]Kothari D Das D Patel S Goyal A 2014 Dextran and Food Application Polysaccharides Ramawat KG Merillon J Springer
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Chyan, W., & Raines, R. T. (2018) · ACS Chemical Biology · doi:10.1021/acschembio.8b00371 · Cited by 180 · see citing works
Self-citationRecent Advances in Nanomicelles Delivery Systems.
Tawfik, S. M., Azizov, S., Elmasry, M. R., Sharipov, M., & Lee, Y.-I. (2020) · Nanomaterials · doi:10.3390/nano11010070 · Cited by 156 · see citing works
“A novel redox-responsive amphiphilic micellar system with a dual function of cancer chemotherapy and bioimaging was developed by Wang’s group [219].”
BackgroundModular Diazo Compound for the Bioreversible Late-Stage Modification of Proteins
Jun, J. V., Petri, Y. D., Erickson, L. W., & Raines, R. T. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c11325 · Cited by 61 · see citing works
Self-citationChen, J., Mei, M., Xu, Y., Xiong, S., Zhao, Y., Liu, R., Shi, S., Wang, H., & Wang, S. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119741 · Cited by 45 · see citing works
Akishiba, M., & Futaki, S. (2019) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.9b00156 · Cited by 41 · see citing works
Peptide-assisted Intracellular Delivery of Biomacromolecules
Futaki, S., Arafiles, J. V. V., & Hirose, H. (2020) · Chemistry Letters · doi:10.1246/cl.200392 · Cited by 34 · see citing works
Mauker, P., Beckmann, D., Kitowski, A., Heise, C., Wientjens, C., Davidson, A. J., Wanderoy, S., Fabre, G., Harbauer, A. B., Wood, W., et al. (2024) · Journal of the American Chemical Society · doi:10.1021/jacs.3c07662 · Cited by 23 · see citing works
Tawfik, S. M., Elmasry, M. R., & Lee, Y. (2019) · Applied Spectroscopy Reviews · doi:10.1080/05704928.2018.1548356 · Cited by 23 · see citing works
Chan, J., Hannah, T., Sadia, S., Kolsteeg, C., Dixon, A., Wiener, R. J., Nguyen, H., Tipping, M. J., Ladeira, J. S., Franklin, P. F. D. C., et al. (2025) · Nature Communications · doi:10.1038/s41467-024-55056-6 · Cited by 17 · see citing works
Polysaccharides for protein and peptide conjugation
Ferguson, E. L., Varache, M., Stokniene, J., & Thomas, D. W. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64081-9.00019-x · Cited by 17 · see citing works
Su, Z., Takeda, Y., Matsui, D., Kogura, T., Toyotake, Y., & Wakayama, M. (2023) · Colloid & Polymer Science · doi:10.1007/s00396-023-05149-3 · Cited by 16 · see citing works
Wintgens, V., Guigner, J., Miskolczy, Z., Amiel, C., & Biczók, L. (2019) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2019.115071 · Cited by 15 · see citing works
Low‐Fouling Zwitterionic Polymeric Colloids as Resuscitation Fluids for Hemorrhagic Shock
Kumar, R., Mancebo, J. G., Patenaude, R. A., Sack, K., Prondzynski, M., Packard, A. B., Dearling, J. L. J., Li, R., Balcarcel‐Monzon, M., Dominguez, S., et al. (2022) · Advanced Materials · doi:10.1002/adma.202207376 · Cited by 11 · see citing works
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Halder, S. K., Delorme, V., & Milner, R. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2510931122 · Cited by 8 · see citing works
Xie, W., He, S., Fang, S., Tian, R., Liang, L., & Wang, D. (2023) · Nanoscale · doi:10.1039/d2nr07280g · Cited by 6 · see citing works
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Shahrbabak, S. M., Tayebi-Khorrami, V., Fadaei, M. R., Moezzi, S. M. I., Hasnain, M. S., Nayak, A. K., & Askari, V. R. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.145980 · Cited by 5 · see citing works
Singh, G., & Singh, N. (2023) · Springer eBooks · doi:10.1007/978-981-99-2196-6_78-1
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Masukawa, M., Duttenhofer, L., Sethi, S., Drees, C., & Walther, A. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.08.20.671219
- Authors
- Chyan, W., Kilgore, H. R., & Raines, R. T.
- Affiliations
- Massachusetts Institute of Technology (US)
- Corresponding author
- Raines, Ronald T.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-04-12
- Volume
- 29
- Issue
- 6
- Pages
- 1942-1949
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- PMC
- PMC6013398
- OpenAlex
- W2797938220
- Cited by
- 20
- References
- 43
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 1
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Bioconjugate Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Funders
- National Institute of General Medical Sciences (R01 GM044783, P41 GM103399)
- National Science Foundation
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- nuclear uptake, receptor-mediated endocytosis, active transport, intracellular drug delivery, polysaccharide conjugates
- MeSH terms
- Biological Transport, Cell Line, Cell Nucleus, Cytosol, Dextrans, Drug Carriers, HeLa Cells, Humans, Models, Molecular
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