The DOI matches its Crossref record; 143 of the 143 references it cites were found in a registry or an index.
A Hitchhiker’s Guide to Click-Chemistry with Nucleic Acids
Open accessAbstract
Click chemistry is an immensely powerful technique for the fast and efficient covalent conjugation of molecular entities. Its broad scope has positively impacted on multiple scientific disciplines, and its implementation within the nucleic acid field has enabled researchers to generate a wide variety of tools with application in biology, biochemistry, and biotechnology. Azide-alkyne cycloadditions (AAC) are still the leading technology among click reactions due to the facile modification and incorporation of azide and alkyne groups within biological scaffolds. Application of AAC chemistry to nucleic acids allows labeling, ligation, and cyclization of oligonucleotides efficiently and cost-effectively relative to previously used chemical and enzymatic techniques. In this review, we provide a guide to inexperienced and knowledgeable researchers approaching the field of click chemistry with nucleic acids. We discuss in detail the chemistry, the available modified-nucleosides, and applications of AAC reactions in nucleic acid chemistry and provide a critical view of the advantages, limitations, and open-questions within the field.
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Cites
Tornøe, C. W., Christensen, C., & Meldal, M. (2002) · The Journal of Organic Chemistry · doi:10.1021/jo011148j · Cited by 8,696
Cu-Catalyzed Azide−Alkyne Cycloaddition
Meldal, M., & Tornøe, C. W. (2008) · Chemical Reviews · doi:10.1021/cr0783479 · Cited by 4,628
Agard, N. J., Prescher, J. A., & Bertozzi, C. R. (2004) · Journal of the American Chemical Society · doi:10.1021/ja044996f · Cited by 3,017
1,3‐Dipolar Cycloadditions. Past and Future
Huisgen, R. (1963) · Angewandte Chemie International Edition in English · doi:10.1002/anie.196305651 · Cited by 2,929
Gratzner, H. G. (1982) · Science · doi:10.1126/science.7123245 · Cited by 2,628
Hein, J. E., & Fokin, V. V. (2010) · Chemical Society Reviews · doi:10.1039/b904091a · Cited by 2,198
Click Chemistry for Drug Development and Diverse Chemical–Biology Applications
Thirumurugan, P., Matosiuk, D., & Jozwiak, K. (2013) · Chemical Reviews · doi:10.1021/cr200409f · Cited by 1,896
A chemical method for fast and sensitive detection of DNA synthesis in vivo
Salic, A., & Mitchison, T. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0712168105 · Cited by 1,861
Copper-free click chemistry for dynamic in vivo imaging
Baskin, J. M., Prescher, J. A., Laughlin, S. T., Agard, N. J., Chang, P. V., Miller, I. A., Lo, A., Codelli, J. A., & Bertozzi, C. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0707090104 · Cited by 1,833
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Cited by
Targeted activation in localized protein environments via deep red photoredox catalysis
Tay, N. E. S., Ryu, K. A., Weber, J. L., Olow, A. K., Cabanero, D. C., Reichman, D. R., Oslund, R. C., Fadeyi, O. O., & Rovis, T. (2023) · Nature Chemistry · doi:10.1038/s41557-022-01057-1 · Cited by 166
Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome
Takhaveev, V., Püllen, N. J. L., Singh, N. K., Lefevre, L., Aghajani, E. A., Huber, S. M., Schauer, S., Gahlon, H. L., Poetsch, A. R., & Sturla, S. J. (2026) · Nature Chemical Biology · doi:10.1038/s41589-025-02052-6 · Cited by 10
Metadata
- Authors
- Fantoni, N. Z., El-Sagheer, A. H., & Brown, T.
- Venue
- Chemical Reviews
- Publisher
- American Chemical Society (ACS)
- Published
- 2021-01-14
- Volume
- 121
- Issue
- 12
- Pages
- 7122-7154
- ISSN
- 0009-2665 (ISSN-L)
- 1520-6890
- OpenAlex
- W3118368175
- Cited by
- 462
- References
- 143
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Fantoni, N. Z., El-Sagheer, A. H., & Brown, T. (2021). A Hitchhiker’s Guide to Click-Chemistry with Nucleic Acids. Chemical Reviews. https://doi.org/10.1021/acs.chemrev.0c00928
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