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Cited by 2,628

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Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA Replication

Gratzner, Howard G.·1982·Science
DOI 10.1126/science.7123245
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Abstract

Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.

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Metadata

Authors
Gratzner, H. G.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1982-10-29
Volume
218
Issue
4571
Pages
474-475
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.7123245
OpenAlex
W2031468048
Cited by
2,628
References
0
FWCI
8.35
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA Replication. https://app.cochat.ai/papers/10.1126/science.7123245
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Gratzner, H. G. (1982). Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA Replication. Science. https://doi.org/10.1126/science.7123245

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.