Inhibition of Quorum Sensing and Virulence in Serratia marcescens by Hordenine
Abstract
Serratia marcescens NJ01 is a pathogenic bacterium isolated from diseased tomato leaves. Here, we report on the development of a tomato- S. marcescens host-pathogen system as a model to evaluate the effects of hordenine on quorum sensing (QS)-mediated pathogenicity under native conditions. Exposure to hordenine at 25, 50, and 100 μg/mL significantly inhibited the production of acyl-homoserine lactones and the formation of biofilms. Hordenine treatment notably enhanced the susceptibility of the preformed biofilms to ciprofloxacin by reducing the production of extracellular polysaccharides, destroying the architecture of biofilms, and changing the permeability of membranes, as evidenced by the scattered appearance and dominant red fluorescence in the combination-treated biofilms. Furthermore, the addition of hordenine affected the production of virulence factors, influenced the intracellular metabolites, and downregulated the expressions of QS- and biofilm-related genes. The plant infection model indicated that hordenine could significantly attenuate the pathogenicity of S. marcescens NJ01 in tomato plants. Thus, hordenine could act as a potential pesticide or pesticide accelerant in treating crop infections.
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Hordenine has MIC 2.5 mg/mL against Serratia marcescens NJ01, the same as against P. aeruginosa PAO1.
“They reported an MIC of 2.5 mg/mL for S. marcescens, which is the same as P. aeruginosa PAO1”
Hordenine reduces biofilm autoinducer levels, inhibits biofilm formation, lessens virulence factors, alters preformed biofilm architecture (higher antibiotic sensitivity with ciprofloxacin), and downregulates QS-related genes in S. marcescens, resembling results with P. aeruginosa PAO1.
“they found results resembling those obtained with P. aeruginosa PAO1, with hordenine reducing biofilm autoinducer levels, inhibiting biofilm formation, lessening virulence factors, altering preformed biofilm architecture (resulting in higher antibiotic sensitivity with ciprofloxacin), and downregulating QS-related genes.”
Hordenine at 25, 50, and 100 μg/mL in LB broth for 24 h reduced C4-HSL levels by 40%, 60%, and 80%, respectively, in S. marcescens.
“and noted that concentrations of 25, 50, and 100 μg/mL of hordenine in an LB broth medium for 24 h resulted in a diminution of C4-HSL levels of 40%, 60%, and 80%, respectively [65].”
Cited by
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Boucher, R., Germain, H., & Desgagné-Penix, I. (2025) · Plants · doi:10.3390/plants14091372 · Cited by 23 · see citing works
Hordenine Protects Against Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Inflammation
Zhang, X., Du, L., Zhang, J., Li, C., Zhang, J., & Lv, X. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.712232 · Cited by 17 · see citing works
- Authors
- Zhou, J.-W., Ruan, L.-Y., Chen, H.-J., Luo, H.-Z., Jiang, H., Wang, J.-S., & Jia, A.-Q.
- Affiliations
- State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry Education, Hainan University, Haikou, Hainan 570228, People’s Republic of China
- School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, People’s Republic of China
- State Key Laboratory of Pharmaceutical Biotechnology (CN)
- Hainan University (CN)
- Corresponding author
- Jia, Ai-Qun
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-01-04
- Volume
- 67
- Issue
- 3
- Pages
- 784-795
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2906958253
- Cited by
- 56
- References
- 0
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 11 · 2023: 15 · 2022: 8 · 2021: 5 · 2020: 8 · 2019: 2
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education of the People's Republic of China (30916011307)
- Ministry of Science and Technology of the People's Republic of China (2017YFD0201401)
- Government of Jiangsu Province
- National Natural Science Foundation of China (41766606)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- Serratia marcescens, hordenine, quorum sensing inhibition, virulence factors, biofilm formation, N-acyl homoserine lactones, tomato leaf disease, host-pathogen model, ciprofloxacin susceptibility, exopolysaccharides, membrane permeability, plant disease control, pesticides
- MeSH terms
- Anti-Bacterial Agents, Bacterial Proteins, Plant Diseases, Serratia marcescens, Tyramine, Virulence, Gene Expression Regulation, Bacterial, Biofilms, Solanum lycopersicum, Virulence Factors, Quorum Sensing, Acyl-Butyrolactones
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