Effects of Immobilized BMP-2 and Nanofiber Morphology on In Vitro Osteogenic Differentiation of hMSCs and In Vivo Collagen Assembly of Regenerated Bone
Abstract
Engineering bone tissue is particularly challenging because of the distinctive structural features of bone within a complex biochemical environment. In the present study, we fabricated poly(L-lactic acid) (PLLA) electrospun nanofibers with random and aligned morphology immobilized with bone morphogenic protein-2 (BMP-2) and investigated how these signals modulate (1) in vitro osteogenic differentiation of human mesenchymal stem cells (hMSCs) and (2) in vivo bone growth rate, mechanical properties, and collagen assembly of newly formed bone. The orientation of adherent cells followed the underlying nanofiber morphology; however, nanofiber alignment did not show any difference in alkaline phosphate activity or in calcium mineralization of hMSCs after 14 days of in vitro culture in osteogenic differentiation media. In vivo bone regeneration was significantly higher in the nanofiber implanted groups (approximately 65-79%) as compared to the defect-only group (11.8 ± 0.2%), while no significant difference in bone regeneration was observed between random and aligned groups. However, nanoindentation studies of regenerated bone revealed Young's modulus and contact hardness with anisotropic feature for aligned group as compared to random group. More importantly, structural analysis of collagen at de novo bone showed the ability of nanofiber morphology to guide collagen deposition. SEM and TEM images revealed regular, highly ordered collagen assemblies on aligned nanofibers as compared…
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BFP-1 modified PLGA scaffold achieved higher osteogenic activity than BMP-2.
“BFP-1 modified PLGA scaffold achieved a higher osteogenic activity than BMP-2”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [85]
BFP-1 modified PLGA scaffold significantly promoted bone formation in a nude murine model.
“the in vivo study further demonstrated that it significantly promoted bone formation in a nude murine model.”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [85]
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Polydopamine-Assisted Surface Modification for Bone Biosubstitutes
Huang, S., Liang, N., Hu, Y., Zhou, X., & Abidi, N. (2016) · BioMed Research International · doi:10.1155/2016/2389895 · Cited by 97 · see citing works
- Authors
- Madhurakkat Perikamana, S. K., Lee, J., Ahmad, T., Jeong, Y., Kim, D.-G., Kim, K., & Shin, H.
- Affiliations
- Incheon National University (KR)
- Division of Orthodontics, College of Dentistry, The Ohio State University, Columbus, Ohio 43210, United States
- The Ohio State University (US)
- Corresponding author
- Kim, Kyobum
- Shin, Heungsoo
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-04-14
- Volume
- 7
- Issue
- 16
- Pages
- 8798-8808
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2054409347
- Cited by
- 68
- References
- 0
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 3 · 2020: 13 · 2019: 13 · 2018: 7 · 2017: 7 · 2016: 7 · 2015: 1
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- The Royal Society of Chemistry’s Journals, Books and Databases (The Royal Society of Chemistry) (submittedVersion)
- Funders
- National Research Foundation of Korea (NRF-2013R1A2A2A03067809)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- BMP-2, poly(L-lactic acid) nanofibers, electrospinning, nanofiber alignment, human mesenchymal stem cells, osteogenic differentiation, bone regeneration, collagen deposition, mechanical anisotropy, nanoindentation, tissue engineering scaffolds
- MeSH terms
- Animals, Biomechanical Phenomena, Cell Adhesion, Cell Differentiation, Collagen, Humans, Mice, Inbred ICR, Osteogenesis, Radiography, Regeneration, Skull, Implants, Experimental, Cell Shape, Bone Morphogenetic Protein 2, Immobilized Proteins, Nanofibers, Mesenchymal Stem Cells
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