Interfacial Self-Assembly of Heparin-Mimetic Multilayer on Membrane Substrate as Effective Antithrombotic, Endothelialization, and Antibacterial Coating
Abstract
In this study, we design the interfacial self-assembly of heparin-mimetic multilayer on poly(ether sulfone) (PES) membrane, which can endow the substrate with excellent cytocompatibility, highly hemocompatibility and enhanced antibacterial properties. The coated 3D sponge-like multilayer was fabricated by surface engineered layer by layer assembly of sulfonic amino polyether sulfone (SNPES) and quaternized chitosan (QC). The cell morphology observation and viability evaluation suggested that the assembled multilayer coating had remarkable cytocompatibility with endothelial cells due to the synergistic promotion of bovine serum albumin adsorption and heparin-mimetic groups; which further indicated that surface endothelialization could be achieved on the heparin-mimetic multilayer. The systematical tests of antithrombotic and blood activation indicated that the heparin-mimetic multilayer-coated membrane owned significantly suppressed adsorption of bovine serum fibrinogen, platelet adhesion and activation, prolonged clotting times, as well as lower activation of blood complement. Furthermore, the antibacterial test suggested the multilayer coated substrates exhibited obvious inhibition capability for both Escherichia coli and Staphylococcus aureus . Therefore, we believe that the developed SNPES/QC multilayer on PES membrane show great potential as a multifunctional coating toward versatile biomedical applications due to the integrated and highly effective antithrombotic, endothelialization, and antibacterial properties.
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Polymers combining multiple functional groups, such as heparin-mimetic or hydrophilic brushes with QAC or Ag nanoparticles, have been tested.
“Polymers that combine multiple functional groups on one surface have also been tested, such as combining synthetic heparin-mimetic polymer or hydrophilic polymer brushes (e.g., PEG) with antibacterial quaternary compounds (QAC) or Ag nanoparticles.105–107”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [105]
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- Authors
- Wang, L., Li, H., Chen, S., Nie, C., Cheng, C., & Zhao, C.
- Affiliations
- Sichuan University (CN)
- Corresponding author
- Cheng, Chong
- Zhao, Changsheng
- Venue
- ACS Biomaterials Science & Engineering
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-10-19
- Volume
- 1
- Issue
- 11
- Pages
- 1183-1193
- ISSN
- 2373-9878 (ISSN-L)
- OpenAlex
- W2515660322
- Cited by
- 33
- References
- 68
- Citations by year
- 2025: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 6 · 2016: 5
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Biomaterials Science & Engineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Sichuan Province (2015TD0001)
- National Natural Science Foundation of China (51225303, 51433007)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Surface Modification and Superhydrophobicity
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- layer-by-layer assembly, polyethersulfone membranes, quaternized chitosan, antithrombotic coatings, endothelialization, antibacterial coatings, hemocompatibility, fibrinogen adsorption, platelet adhesion, Escherichia coli, Staphylococcus aureus, cytocompatibility, endothelial cells, bovine serum albumin adsorption
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