The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay
Abstract
The lateral flow assay is one of the most convenient analytical techniques for analyzing the immune response, but its applicability to precise genetic analyses is limited by the false-positive signal and tedious and inefficient hybridization steps. Here, we introduce the CRISPR (clustered regularly interspaced short palindromic repeats) /Cas system into the lateral flow assay, termed CRISPR/Cas9-mediated lateral flow nucleic acid assay (CASLFA), to address such issues. In this study, CASLFA is utilized to identify Listeria monocytogenes, genetically modified organisms (GMOs), and African swine fever virus (ASFV) at a detection limit of hundreds of copies of genome samples with high specificity within 1 h. We further evaluated the performance of CASLFA in a nonlaboratory environment and successfully confirmed 27 ASFV-infected samples from 110 suspected swine serum samples, with an accuracy of 100% when compared to real-time PCR (RT-PCR) assay. CASLFA satisfies some of the characteristics of a next-generation molecular diagnostics tool due to its rapidity and accuracy, allowing for point-of-care use without the need for technical expertise and complex ancillary equipment. This method has great potential for gene analysis in resource-poor or nonlaboratory environments.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aar6245 · Cited by 4,507
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
Hu, J. H., Miller, S. M., Geurts, M. H., Tang, W., Chen, L., Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., et al. (2018) · Nature · doi:10.1038/nature26155 · Cited by 1,552
Cited by
Diagnosing COVID-19: The Disease and Tools for Detection
Udugama, B., Kadhiresan, P., Kozlowski, H. N., Malekjahani, A., Osborne, M., Li, V. Y. C., Chen, H., Mubareka, S., Gubbay, J. B., & Chan, W. C. W. (2020) · ACS Nano · doi:10.1021/acsnano.0c02624 · Cited by 1,754
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis
Liu, Y., Zhan, L., Qin, Z., Sackrison, J., & Bischof, J. C. (2021) · ACS Nano · doi:10.1021/acsnano.0c10035 · Cited by 626
Choi, J.-H., Lim, J., Shin, M., Paek, S.-H., & Choi, J.-W. (2021) · Nano Letters · doi:10.1021/acs.nanolett.0c04303 · Cited by 382
Lateral flow test engineering and lessons learned from COVID-19
Budd, J., Miller, B. S., Weckman, N. E., Cherkaoui, D., Huang, D., Decruz, A. T., Fongwen, N., Han, G.-R., Broto, M., Estcourt, C. S., et al. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-022-00007-3 · Cited by 308
Metadata
- Authors
- Wang, X., Xiong, E., Tian, T., Cheng, M., Lin, W., Wang, H., Zhang, G., Sun, J., & Zhou, X.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-02-11
- Volume
- 14
- Issue
- 2
- Pages
- 2497-2508
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W3006168659
- Cited by
- 326
- References
- 3
- FWCI
- 16.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wang, X., Xiong, E., Tian, T., Cheng, M., Lin, W., Wang, H., Zhang, G., Sun, J., & Zhou, X. (2020). Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay. ACS Nano. https://doi.org/10.1021/acsnano.0c00022
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