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Cited by 43Extracellular vesicles in diseaseRNA Interference and Gene DeliveryNanoparticle-Based Drug Delivery

Customized Loading of microRNA-126 to Small Extracellular Vesicle-Derived Vehicles Improves Cardiac Function after Myocardial Infarction

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Bheri, Sruti, Brown, Milton E., Park, Hyun-Ji, Brazhkina, Olga, Takaesu, Felipe, Davis, Michael E.·2023·ACS Nano
DOI 10.1021/acsnano.3c01534PMC PMC10604069
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Abstract

High Resolution Image Download MS PowerPoint Slide Small extracellular vesicles (sEVs) are promising for cell-based cardiac repair after myocardial infarction. These sEVs encapsulate potent cargo, including microRNAs (miRs), within a bilayer membrane that aids sEV uptake when administered to cells. However, despite their efficacy, sEV therapies are limited by inconsistencies in the sEV release from parent cells and variability in cargo encapsulation. Synthetic sEV mimics with artificial bilayer membranes allow for cargo control but suffer poor stability and rapid clearance when administered in vivo . Here, we developed an sEV-like vehicle (ELV) using an electroporation technique, building upon our previously published work, and investigated the potency of delivering electroporated ELVs with pro-angiogenic miR-126 both in vitro and in vivo to a rat model of ischemia–reperfusion. We show that electroporated miR-126+ ELVs improve tube formation parameters when administered to 2D cultures of cardiac endothelial cells and improve both echocardiographic and histological parameters when delivered to a rat left ventricle after ischemia reperfusion injury. This work emphasizes the value of using electroporated ELVs as vehicles for delivery of select miR cargo for cardiac repair.

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  • EVs cannot be stored for a long time since they lose their structure and bioactivity due to destruction of the lipid layers.

    “However, we cannot store the EVs for a long time since they lose their structure and bioactivity due to the destruction of the lipid layers [327–329].”

    From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [329]

    The Experimental Methods (Isolation and Characterization of sEVs; Synthesis of miR-Loaded ELVs with Electroporation; page numbers unavailable) say the vesicles were stored at −80 °C until use, but give no storage duration or evidence of deterioration. A search for long-term storage, loss of bioactivity, and lipid-bilayer damage found no passage addressing the claimed cause of storage limits. (LLM, conf=0.72)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Bheri, S., Brown, M. E., Park, H.-J., Brazhkina, O., Takaesu, F., & Davis, M. E.
Affiliations
Georgia Institute of Technology (US)
Corresponding author
Davis, Michael E.
ORCID
Venue
ACS Nano
Publisher
American Chemical Society (ACS)
Published
2023-09-16
Volume
17
Issue
20
Pages
19613-19624
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
DOI
10.1021/acsnano.3c01534
PMC
PMC10604069
OpenAlex
W4386800820
Cited by
43
References
1
Citations by year
2026: 18 · 2025: 14 · 2024: 10 · 2023: 1
FWCI
3.83
Type
journal article
Language
en
Open access
Licence
Locations
  • ACS Nano (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (R01 HL145644)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords
miR-126, small extracellular vesicles, electroporation, myocardial infarction, cardiac repair, angiogenesis, endothelial tube formation, ischemia-reperfusion injury, microRNA delivery, cell-free therapy
MeSH terms
Extracellular Vesicles, Animals, Ischemia, Myocardial Infarction, MicroRNAs, Endothelial Cells, Rats

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