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Cited by 25Advanced Fluorescence Microscopy TechniquesMolecular Sensors and Ion Detectionbioluminescence and chemiluminescence research

Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life

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Jensen, Gary C., Janis, Makena K., Nguyen, Hazel N., David, Ogonna W., Zastrow, Melissa L.·2024·ACS Sensors
DOI 10.1021/acssensors.3c02695PMC PMC11073808
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Abstract

Genetically encoded fluorescent metal ion sensors are powerful tools for elucidating metal dynamics in living systems. Over the last 25 years since the first examples of genetically encoded fluorescent protein-based calcium indicators, this toolbox of probes has expanded to include other essential and non-essential metal ions. Collectively, these tools have illuminated fundamental aspects of metal homeostasis and trafficking that are crucial to fields ranging from neurobiology to human nutrition. Despite these advances, much of the application of metal ion sensors remains limited to mammalian cells and tissues and a limited number of essential metals. Applications beyond mammalian systems and in vivo applications in living organisms have primarily used genetically encoded calcium ion sensors. The aim of this Perspective is to provide, with the support of historical and recent literature, an updated and critical view of the design and use of fluorescent protein-based sensors for detecting essential metal ions in various organisms. We highlight the historical progress and achievements with calcium sensors and discuss more recent advances and opportunities for the detection of other essential metal ions. We also discuss outstanding challenges in the field and directions for future studies, including detecting a wider variety of metal ions, developing and implementing a broader spectral range of sensors for multiplexing experiments, and applying sensors to a wider range of single- and multi-species biological systems.

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  • It is becoming possible to better visualize metals in motion with sensitive analytical tools, including an ever-increasing set of sensors to monitor chelatable metal ions in living cells.

    “Together with sensitive analytical tools, including an ever-increasing set of sensors to monitor chelatable metal ions in living cells,223−225 it is becoming possible to better visualize metals in motion.”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [223]

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Cited by

Sorted by citations

Authors
Jensen, G. C., Janis, M. K., Nguyen, H. N., David, O. W., & Zastrow, M. L.
Affiliations
University of Houston (US)
Corresponding author
Zastrow, Melissa L.
ORCID
Venue
ACS Sensors
Publisher
American Chemical Society (ACS)
Published
2024-04-08
Volume
9
Issue
4
Pages
1622-1643
ISSN
2379-3694 (ISSN-L)
DOI
10.1021/acssensors.3c02695
PMC
PMC11073808
OpenAlex
W4394580513
Cited by
25
References
3
Citations by year
2026: 6 · 2025: 17 · 2024: 2
FWCI
1.29
Type
journal article
Language
en
Open access
Licence
Locations
  • ACS Sensors (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Welch Foundation (E-2176-20230405)
  • U.S. Department of Health and Human Services (R35 GM138223)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
Keywords
metal ion sensors, calcium sensor, metal homeostasis, metal trafficking, in vivo imaging, multiplexed sensing
MeSH terms
Animals, Calcium, Fluorescent Dyes, Humans, Ions, Luminescent Proteins, Metals, Biosensing Techniques

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