The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
Abstract
We report on and provide a detailed characterization of the performance and properties of a recently developed, all Escherichia coli, cell-free transcription and translation system. Gene expression is entirely based on the endogenous translation components and transcription machinery provided by an E. coli cytoplasmic extract, thus expanding the repertoire of regulatory parts to hundreds of elements. We use a powerful metabolism for ATP regeneration to achieve more than 2 mg/mL of protein synthesis in batch mode reactions, and more than 6 mg/mL in semicontinuous mode. While the strength of cell-free expression is increased by a factor of 3 on average, the output signal of simple gene circuits and the synthesis of entire bacteriophages are increased by orders of magnitude compared to previous results. Messenger RNAs and protein degradation, respectively tuned using E. coli MazF interferase and ClpXP AAA+ proteases, are characterized over a much wider range of rates than the first version of the cell-free toolbox. This system is a highly versatile cell-free platform to construct complex biological systems through the execution of DNA programs composed of synthetic and natural bacterial regulatory parts.
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Cites
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Cited by
Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity
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Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
Reconstituting Natural Cell Elements in Synthetic Cells
Gaut, N. J., & Adamala, K. P. (2021) · Advanced Biology · doi:10.1002/adbi.202000188 · Cited by 92
From protocells to prototissues: a materials chemistry approach
Gobbo, P. (2020) · Biochemical Society Transactions · doi:10.1042/bst20200310 · Cited by 35
Alessa, O., Aiba, Y., Arbaah, M., Hidaka, Y., Watanabe, S., Miyanaga, K., Wannigama, D. L., & Cui, L. (2025) · Molecules · doi:10.3390/molecules30153132 · Cited by 21
Metadata
- Authors
- Garamella, J., Marshall, R., Rustad, M., & Noireaux, V.
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-02-09
- Volume
- 5
- Issue
- 4
- Pages
- 344-355
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2337003117
- Cited by
- 419
- References
- 67
- FWCI
- 20.16
- Type
- journal article
- Language
- en
- Open access
- closed
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- Garamella, J., Marshall, R., Rustad, M., & Noireaux, V. (2016). The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.5b00296
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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