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A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield
Abstract
A cell-free translation system has been constructed that uses a continuous flow of the feeding buffer [including amino acids, adenosine triphosphate (ATP), and guanosine triphosphate (GTP)] through the reaction mixture and a continuous removal of a polypeptide product. Both prokaryotic ( Escherichia coli ) and eukaryotic (wheat embryos, Triticum sp. ) versions of the system have been tested. In both cases the system has proven active for long times, synthesizing polypeptides at a high constant rate for tens of hours. With the use of MS2 phage RNA or brome mosaic virus RNA 4 as templates, 100 copies of viral coat proteins per RNA were synthesized for 20 hours in the prokaryotic or eukaryotic system, respectively. With synthetic calcitonin messenger RNA, 150 to 300 copies of calcitonin polypeptide were produced per messenger RNA in both types of continuous translation systems for 40 hours.
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Cited by
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Noireaux, V., & Libchaber, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408236101 · Cited by 1,152
Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
An integrated cell‐free metabolic platform for protein production and synthetic biology
Jewett, M. C., Calhoun, K. A., Voloshin, A., Wuu, J. J., & Swartz, J. R. (2008) · Molecular Systems Biology · doi:10.1038/msb.2008.57 · Cited by 384
Development of an artificial cell, from self-organization to computation and self-reproduction
Noireaux, V., Maeda, Y. T., & Libchaber, A. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1017075108 · Cited by 340
Programmable on-chip DNA compartments as artificial cells
Karzbrun, E., Tayar, A. M., Noireaux, V., & Bar-Ziv, R. H. (2014) · Science · doi:10.1126/science.1255550 · Cited by 264
Metadata
- Authors
- Spirin, A. S., Baranov, V. I., Ryabova, L. A., Ovodov, S., & Alakhov, Y. B.
- Venue
- Science
- Published
- 1988-11-25
- Volume
- 242
- Issue
- 4882
- Pages
- 1162-1164
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2062876104
- Cited by
- 599
- References
- 0
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Spirin, A. S., Baranov, V. I., Ryabova, L. A., Ovodov, S., & Alakhov, Y. B. (1988). A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield. Science. https://doi.org/10.1126/science.3055301
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