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Cellulose Nanocrystal/Gold Nanoparticle Composite as a Matrix for Enzyme Immobilization
Open accessAbstract
A novel nanocomposite consisting of cellulose nanocrystals (CNCs) functionalized with gold nanoparticles (AuNPs) serving as an excellent support for enzyme immobilization with phenomenally high loading is presented in this work. As testing models, cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase were conjugated on an activated CNC/AuNP matrix. This catalytic platform exhibits significant biocatalytic activity with excellent enzyme stability and without apparent loss of the original activity. The recovered specific activities were approximately 70% and 95% for CGTase and alcohol oxidase, respectively. This novel and inexpensive material is anticipated to extend to other enzymes, enhancing the enzyme loading and activity as well as the stability in both operation and storage.
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Beck-Candanedo, S., Roman, M., & Gray, D. G. (2005) · Biomacromolecules · doi:10.1021/bm049300p · Cited by 1,588
Polymer nanocomposites reinforced by cellulose whiskers
Favier, V., Chanzy, H., & Cavaille, J. Y. (1995) · Macromolecules · doi:10.1021/ma00122a053 · Cited by 1,149
Cited by
Production of nanocrystalline cellulose from lignocellulosic biomass: Technology and applications
Brinchi, L., Cotana, F., Fortunati, E., & Kenny, J. M. (2013) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2013.01.033 · Cited by 1,121
Potential applications of enzymes immobilized on/in nano materials: A review
Ansari, S. A., & Husain, Q. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.09.005 · Cited by 1,118
Peng, B. L., Dhar, N., Liu, H. L., & Tam, K. C. (2011) · The Canadian Journal of Chemical Engineering · doi:10.1002/cjce.20554 · Cited by 890
Varma, R. S. (2019) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.8b06550 · Cited by 363
Mahmoud, K. A., Mena, J. A., Male, K. B., Hrapovic, S., Kamen, A., & Luong, J. H. T. (2010) · ACS Applied Materials & Interfaces · doi:10.1021/am1006222 · Cited by 316
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Metadata
- Authors
- Mahmoud, K. A., Male, K. B., Hrapovic, S., & Luong, J. H. T.
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-07-07
- Volume
- 1
- Issue
- 7
- Pages
- 1383-1386
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2013644730
- Cited by
- 190
- References
- 2
- FWCI
- 2.76
- Type
- journal article
- Language
- en
- Open access
- green
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